KRASTANOV, A., A. GOVINDARAJAN and D. DANIEL, 2008. Studies on lipase fermentation using Candida cylindracea NRRL Y-17506 in a stired tank bioreactor. Bulg. J. Agric. Sci., 14: 290-299 Lipase is an enzyme of considerable commercial and industrial importance. The major applications of lipase are in the fat and oleochemical, food and dairy, textile, detergent, cosmetics, tea processing, pulp and ...

The aim of this study was to determine whether the increase in lipoprotein lipase activity displayed by the adipose tissue of obese (Ja/fa) rats as compared with that of lean (Fa/fa) rats could be ascribed to a change in the content or in the catalytic properties of the enzyme. The question was addressed in rats of two ages: in 7-day-old suckling and in 30-day-old post-weaning pups. Inguinal fa...

This study was aimed at producing protease and lipase simultaneously on a common medium by Bacillus licheniformis VSG1, which was isolated from a tannery effluent. The effect of media composition with respect to protein source, lipid source and emulsifier on the production of protease and lipase was analysed. Both those enzymes were produced under optimized conditions like pH, temperature and i...

Lipoprotein lipase (LP lipase, triacylglycero-protein acylhydrolase EC 3.1.1.34) activity was found in four dissimilar brain regions (hypothalamus, cortex, cerebellum, and midbrain) of adult male rats. Progressive accumulation of LP lipase activity in cultured fetal rat hypothalamic cells was also observed, indicating de novo synthesis of the lipase. The brain LP lipase activity was serum-depen...

The first indication for the existence of a cofactor for pancreatic lipase was reported in 1910 by Rosenheim (1 ) . This paper is of some historical interest and contains the following statement: “It seems therefore that pancreatic lipase is a complex enzyme which can be separated into two inactive fractions by means of filtration, a fact which has so far not been observed in any other enzyme”....

Lipase biosynthesis optimization, Aspergillus niger ADM 110, Gamma radiation Objectives: The present study describes the isolation, identification and screening of fugal isolates for the biosynthesis of extracellular lipase enzyme. Methodology: Thirteen fungal cultures were isolated from cotton seeds, waste cake of cotton seed oil and spent bleaching earth, samples by serial dilution method. Al...

A triacylglycerol lipase (EC 3.1.1.3) from the conidia of Neurospora crassa was purified and characterized. The enzyme was purified by Sephadex G-100 column chromatography. Homogeneity was checked by PAGE, and isoelectric focusing gave a single band corresponding to a pI of 6.4. The enzyme had an apparent Mr 54000 +/- 1000 as determined by gel filtration. SDS-PAGE gave a single band of Mr 27000...

Endoglucanase and lipase showed good deinking efficiency for waste papers. The performances could be greatly improved further by the combined use of the two enzymes. To reduce the enzyme production cost and enhance synergistic action of endoglucanase and lipase on laser-printed paper and newspaper, a chimeric enzyme with endoglucanase and lipase activity was constructed and expressed in Pichia ...