Colicins kill Escherichia coli after translocation across the outer membrane. Colicin N displays an unusually simple translocation pathway, using the outer membrane protein F (OmpF) as both receptor and translocator. Studies of this binary complex may therefore reveal a significant component of the translocation pathway. Here we show that, in 2D crystals, colicin is found outside the porin trim...

The outer membranes (OMs) of gram-negative bacteria have an asymmetric lipid distribution with lipopolysaccharides at the outer leaflet and phospholipids (PLs) at the inner leaflet. This lipid arrangement is essential for the barrier function of the OM and for the viability of most gram-negative bacteria. Cells with OM assembly defects or cells exposed to harsh chemical treatments accumulate PL...

objective(s):pharmacological studies showed that the extracts of jin yin hua and its active constituents have lipid lowering, antipyretic, hepatoprotective, cytoprotective, antimicrobial, antibiotic, antioxidative, antiviral, and anti-inflammatory effects. the purpose of the present study was to investigate the protective effects of caffeoylquinic acids (cqas) from jin yin hua against hydrogen ...

To explore the effect of Panax notoginseng saponins on atherosclerosis with type 2 diabetes mellitus in Goto-Kakizaki rats by nuclear factor-kappa B signaling pathway. fed high fat diet and injected N-nitro-L-arginine methyl ester for 8 w, were randomized into model group (400 mg/kg/d). Another Wistar selected as control which normal saline. Serum lipid inflammatory factors measured. There no s...

The somatic genetic defect in paroxysmal nocturnal haemoglobinuria (PNH) involves a block in the transfer of GlcNAc from UDP-GlcNAc to phosphatidylinositol (PI), the first step in the biosynthetic pathway for glycosylphosphatidylinositols (GPIs). We asked whether an exogenous lipid corresponding to an early intermediate in this pathway can be taken up by cells in culture and proceed through the...

Extracellular vesicles (EVs) are a heterogeneous population of stable lipid membrane particles that play critical role in the regulation numerous physiological and pathological processes. EV cargo, which includes lipids, proteins, RNAs including miRNAs, is affected by metabolic status parental cell. Concordantly, abnormalities autophagic-endolysosomal pathway, as seen lysosomal storage disorder...

Single cell oil (SCO) produced by yeast is an attractive alternative due to higher yield of lipid in a limited space with opportunity for naturally manipulating its quality. In the present study, banana peel used as source carbon biotransformation Rhodotorula mucilaginosa lipid. Further, quality and quantity are enhanced using discarded aqueous Prosopis cineraria pod extract natural inhibitor. ...

Fatty acids are essential components of the dynamic lipid metabolism in cells. Fatty acids can also signal to intracellular pathways to trigger a broad range of cellular responses. Oleic acid is an abundant monounsaturated omega-9 fatty acid that impinges on different biological processes, but the mechanisms of action are not completely understood. Here, we report that oleic acid stimulates the...

Inositol phosphates (InsPs) and diacyglycerol (DAG) are second messengers derived via the breakdown of inositol phospholipids, and which play important signalling roles in the regulation of proliferation of some cell types. We have studied the operation of this pathway during the early stages of retionic acid (RA)-induced granulocytic differentiation of HL60 myeloid leukemia cells. The autonomo...

The most widely used plasticizer, di-(2-ethylhexyl) phthalate (DEHP), is known to affect lipid metabolism and adipogenesis. We studied the effects of dietary DEHP exposure on metabolism in rats as well as the role of the JAK/STAT pathway in this process. Eighty rats were exposed to DEHP (0, 5, 50 and 500 mg/kg/d) through dietary intake for 4 weeks. We then collected blood samples, liver, and ad...