The effect of Vespa amino acid mixture (VAAM) on the release of lipolytic products was examined in isolated rat adipocytes. Concentrations of 112.5 to 225 ppm of VAAM showed significantly greater release of non-esterified fatty acids (NEFA) and glycerol than the same concentrations of casein amino acid mixture (CAAM). The integrated relative release of NEFA and glycerol was lower in response to...

Three lipase-producing thermophilic bacteria (AK-P1, AK-P2, and AK-P3) were isolated from the Taptapani hot water spring in Orissa, India. The crude extra cellular lipases from cell-free culture supernatant were reacted in an olive oil mixture, and their lipolytic activities were compared. Identification of the bacteria was carried out using biochemical tests, 16SrRNA sequencing and sequences s...

Cholera toxin, in contrast to its stimulatory action on adenylate cyclase, renders the insulin-stimulated adipose cell insensitive to the inhibitory actions of lipolytic agents such as isoprenaline, adrenocorticotropic hormone or glucagon. Conversely, pertussis toxin eliminates both the stirnulatory actions of the antilipolytic agents, such as adenosine, nicotinic acid, and prostaglandin E , , ...

1. Adipocytes isolated from rats 6-9 days after adrenalectomy had significantly increased sensitivity to insulin action against noradrenaline-stimulated lipolysis. In the presence of adenosine deaminase there was no significant difference in insulin sensitivity between cells from adrenalectomized and sham-operated rats. 2. Adipocytes from adrenalectomized rats had decreased lipolytic responses ...

Davis, B. R. (Communicable Disease Center, Atlanta, Ga.), and W. H. Ewing. Lipolytic, pectolytic, and alginolytic activities of Enterobacteriaceae. J. Bacteriol. 88:16-19. 1964.-Of 2,262 cultures of Enterobacteriaceae tested, only cultures of Enterobacter liquefaciens (Aerobacter liquefaciens), Serratia, Proteus vulgaris, and P. mirabilis gave evidence of lipolytic activities. None of the cultu...

The anti-lipolytic effect of the adenosine analogue N6-L-phenylisopropyladenosine was studied with rat adipocytes incubated with a high concentration of adenosine deaminase (0.5 unit/ml, approx. 2.5 micrograms/ml) and concentrations of noradrenaline that were equieffective in different physiological states. These studies were performed to compare the fed and starved (24h) states and to compare ...

高折 修二 ・島本 暉朗 The experimental atheromatosis in various animals fed on high-fat, high-cholesterol diet has widely been used for the elucidation of the pathogenesis of clinical arterioscle rosis and for the laboratory evalution of lipolytic agents. Page et al. (1) and Myasnikov (2) have shown that rabbits are highly susceptive to the exogenously administered cho lesterol, while rats are relativel...

It has been proposed that differences in adipocyte function and/or metabolism between obese and lean individuals may manifest themselves in functional adipose tissue abnormalities that lead to metabolic disorders in obesity. We studied lipogenesis and lipolysis of omental adipocytes from obese (OB) and non-obese (NOB) humans. The specific activity of the lipogenic marker enzyme G3PDH was 50% lo...