A liquid chromatography/mass spectrometry (LC/MS) method using desorption electrospray ionization (DESI) as a versatile interface has been established, which allows a wide range of elution flow rates, online derivatization via reactive DESI and further combination with electrochemistry.

Monomeric and oligomeric proanthocyanidins present in a range of plant-derived foods and beverages were separated by degree of polymerization and identified using a modified normal-phase high-performance liquid chromatography (HPLC) method coupled with on-line mass spectrometry (MS) analysis using an atmospheric pressure ionization electrospray chamber. In addition, ultraviolet (UV) and fluores...

We developed a rapid, simple and sensitive LC/MS/MS method for the simultaneous quantitation of tegafur (FT) and gimeracil (CDHP) in human plasma with a concentration range of 20-5000 and 2-500 ng/mL, respectively. Methanol was chosen as a precipitation agent for sample preparation. Chromatographic separation was performed on an inertsil ODS-3 C18 column using 1.0% formic acid in water and meth...

This paper studies the effect of photosensitizer (acetone, H(2)O(2), surfactant and pigment) on photodegradation of diclofenac (DCF) under simulated sunlight. The results demonstrate that degradation pathways proceed via pseudo first-order kinetics in all cases. The photodegradation rate was found to increase with increasing acetone and H(2)O(2). Surfactant and pigment inhibited the photodegrad...

Turbulent-flow chromatography (TFC) on-line coupled to liquid chromatography mass spectrometry (LC-MS) is used to determine flavonoids and resveratrol in different types of wines. A fully automated system was developed in which 10 mL of sample (diluted wine) was passed over a TFC column, after which the retained analytes were separated by reversed-phase LC and detected by negative ion mode atmo...

A label-free quantification strategy including the development of in-house software (NakedQuant) to calculate the average TIC across all spectral counts in tandem affinity purification (TAP)-tagging liquid chromatography-mass spectrometry MS/MS (LC/MS/MS) experiments was applied to a large-scale study of protein complexes in the MAPK portion of the insulin signaling pathway from Drosophila cell...

We describe the preparation of [2,4,16,16-2H4]estradiol-17 beta. The method for quantitative determination of subpicomole amounts of estradiol-17 beta (trimethylsilyl ether derivatives) by gas-liquid chromatography-mass spectrometry with multiple ion detector and with [2H4]estradiol-17 beta as the carrier is presented and characterized. Values for serum samples, as measured by radioimmunoassay ...

Platycodin D (PD), a major component isolated from the root of Platycodon grandiflorum, is widely used in traditional Chinese medicine. A sensitive rapid analytical method was established and validated to determine the PD in rat plasma. This method was further applied to assess the pharmacokinetics of PD in rats following administration of a single dose. Liquid chromatography tandem mass spectr...

Proteomics promises to revolutionize cancer treatment and prevention by facilitating the discovery of molecular biomarkers. Progress has been impeded, however, by the small-sample, high-dimensional nature of proteomic data. We propose the application of a Bayesian approach to address this issue in classification of proteomic profiles generated by liquid chromatography-mass spectrometry (LC-MS)....