White-rot fungi secret a large number of hydrolytic and oxidative enzymes for degradation of lignocellulosic material. The sequencing of the genome of the white-rot fungus Phanerochaete chrysosporium has facilitated the characterization of its complete extracellular proteome. P. chrysosporium was grown on liquid medium, containing glucose, cellulose or wood chips as the carbon source, and also ...

BACKGROUND AND OBJECTIVE Myelodysplastic syndrome progenitor cells can be grown and expanded in long term bone marrow liquid cultures in the presence of multiple cytokines. In this study we investigated the pattern of differentiation and response to growth factors in six cases of myelodysplastic syndrome (MDS) with well-defined cytogenetic abnormalities by means of conventional cytogenetics and...

In this study, the degradation of metalaxyl was investigated in the presence of two Mucorales strains, previously isolated from soil subjected to repeated treatments with this fungicide and selected after enrichment technique. Fungal strains were characterised by a polyphasic approach using phylogenetic analysis of the Internal Transcribed Spacer (ITS) gene region, phenotypic characterisation b...

Haynes, W. C. (Northern Regional Research Laboratory, Peoria, Ill.), and Lenora J. Rhodes. Spore formation by Bacillus popilliae in liquid medium containing activated carbon. J. Bacteriol. 91:2270-2274. 1966.-Heretofore, it has not been found possible to evoke sporulation of Bacillus popilliae in liquid culture. We have discovered that sporulation will occur in tryptone-glucose-yeast extract br...

Spent culture supernatant from early-stationary-phase Mycobacterium tuberculosis H37Ra cultures increased the viability of bacilli from aged cultures of this strain and allowed small inocula to initiate growth in liquid culture. The resuscitation factor was acid labile and heat stable, with a mass of less than 1,375 Da.

The maximum temperature for growth (T(max)) was determined for pure and mixed cultures of acidophilic thiobacilli. The experimental system was based on incubating the cultures in liquid media exposed to a linear temperature gradient. The T(max) values varied within the range of 36.1 to 43.6 degrees C.

Bone-marrow was aspirated into 10 ml Eagle's minimal essential medium (EMEM) containing preservative-free heparin. After washing, the samples were divided into four parts; A for tube cultures,. B for coverslip cultures in multidish wells; C was placed on a 'Ficoll'-'Hypaque' gradient to obtain free mononuclear cells, and D was frozen in liquid nitrogen in cryopreservative medium containing 1% d...

Megakaryocytopoiesis and granulopoiesis of marrow cells from W/Wv mice were studied using a continuous liquid marrow culture system. Cells in the suspension phase were assayed weekly over a 16-week period for total nucleated cells, megakaryocytes, granulocytes, megakaryocytes and granulocyte-macrophage progenitor cells (CFU-Ms, CFU-GMs), and spleen colony-forming cells (CFU-Ss). Without hydroco...