Putative standards of skeletal alkaline phosphatase (ALP) (from bone, bone cells, osteosarcoma cells, and Pagetic serum) and hepatic ALP (from cholestatic serum and bile) were used to compare three methods for quantifying skeletal ALP activity in serum: heat inactivation, precipitation with wheat germ agglutinin (WGA), and precipitation with concanavalin A (Con A). All the skeletal ALP standard...

BACKGROUND Serum alkaline phosphatase (ALP) level has been shown to be a prognostic factor for myocardial infarction and stroke via its promotion of vascular calcification. AIM To investigate for the first time the correlation between serum ALP level and coronary collateral circulation (CCC) development. METHODS A total of 356 patients with stable angina pectoris were evaluated retrospectiv...

The fluctuations in the plasma levels of several bone markers were investigated in newborn calves. Experiment 1 monitored the postnatal changes in the plasma levels of tartrate-resistant acid phosphatase isoform 5b (TRAP5b), total alkaline phosphatase (t-ALP) and bone-specific alkaline phosphatase (BAP) in four calves. These markers increased significantly from 9-20 hr after the first colostrum...

conclusions the elevation of hmw-alp activity in cu treated animal suggests the occurrence of biliary disease. this may be used as a biomarker for the diagnosis of copper toxicity. results obtained data showed that with increasing administration of copper, the alp activity was elevated significantly. in comparison with the control group the elevations were between 20%-56% using gel filtration c...

We propose a novel electrochemical detection system for alkaline phosphatase (ALP) activity using the difference in water and oil solubilities between the substrate, ferrocene ethyl phosphate ester (FcEtOPO(3)(2-)), and the enzymatic product, ferroceneethanol (FcEtOH). In this system, water droplets containing ALP and FcEtOPO(3)(2-) were placed on a Pt disk microelectrode and surrounded by a mi...

Abstract Alkaline phosphatase (ALP) is an important marker for many diseases, yet efficient and reliable detection method still lacking. Here, we present a novel photonic alginate hydrogel particle as ALP sensor based on the competitive combination of intermediary agent, pyrophosphate ion (PPi), with crosslinker, which can trigger phase-transition response from hydrogel. This be converted into ...

To characterize serum constituents in blood in response to cold exposure, we measured serum inorganic phosphate (Pi) and alkaline phosphatase (ALP) levels. After 30 min exposure of cold, the serum Pi and alkaline phosphatase levels were 3.4 ± 0.06 mg/l and 72.33 ± 0.45 μmole/l respectively where as the control levels of these constituents are 3.05 ± 0.03 mg/l and 84 μmole/l respectively. Cold e...

Fractionation of serum alkaline phosphatase by either biochemical or electrophoretic techniques is often insufficient to determine the source of the elevated serum enzyme. In 31 unselected patients with high serum alkaline phosphatase a simultaneous determination of serum gamma glutamyl transpeptidase and urinary hydroxyproline excretion rates was performed, in addition to urea denaturation and...

Serum gamma-glutamyl transpeptidase ( G G T ), alkaline phosphatase (A L P ) and aspartate aminotransferase (A ST) were assayed in patients with cancer. GGT was more sensitive than either ALP or AST in detecting metastatic car­ cinoma to the liver in both icteric and anicteric patients with cancer. Extremely high activities of GGT were seen in patients with hepatoma, carcinoma of the pancreas a...

The objective of this study was to determine the activities lactate dehydrogenase (LDH), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), aspartate aminotransferase (AST) and alanine (ALT) in milk blood lactating Holstein cows investigate their association with subclinical mastitis (SCM). This also aims evaluate impact SCM its severity based on California test (CMT) scores above me...