The extracellular domain of the human epidermal growth factor receptor (sEGFR) consists of 621 amino acid residues, including 50 cysteines. The connections of the 25 disulfide bonds in the recombinant sEGFR protein, obtained from Chinese hamster ovary cells, have been determined using N-terminal sequencing and matrix-assisted laser desorption/ionization mass spectroscopy. We identified a basic ...

Laminins are extracellular matrix glycoproteins that influence the phenotype and functions of many types of cells. Laminins are heterotrimers composed of a, b, and g polypeptides. So far five a, three b, and two g polypeptide chains, and 11 variants of laminins have been proposed. Laminins interact in vitro with mature blood cells and malignant hematopoietic cells. Most studies have been perfor...

To examine the ultrastructural distribution of laminin within kidney basement membranes, we prepared rat anti-mouse laminin mAbs to use in immunolocalization experiments. Epitope domains for these mAbs were established by immunoprecipitation, immunoblotting, affinity chromatography, and rotary shadow EM. One mAb bound to the laminin A and B chains on blots and was located to a site approximatel...

Modulating the extracellular matrix microenvironment is critical for achieving desired macrophage phenotype in immune investigations or tumor therapy. Combining de novo protein design and biosynthesis techniques, herein, we designed a biomimetic polypeptide self-assembled nano-immunomodulator to trigger activation of specific phenotype. It was intended be made up (​GGS​GGP​GGG​PAS​AAA​NSA​SRA​T...

in a structural analysis, the “rebellion & decline” theme creates a narrative structure in which eleven stories of shāhnāmeh – equal to 3.18 per cent of the whole epic – are included, which makes it the tenth largest sequence of the shāhnāmeh. this narrative structure (sequence) consists of initial tranquillity, the king’s rebellion, his decline, and ultimate tranquillity. the epical theme of r...