A 32-year-old woman presented with an 8-month history of verrucous and suppurative skin lesions on the anogenital area. She was diagnosed with human immunodeficiency virus (HIV) infection 18 months before and was receiving antiretroviral therapy thereafter. She underwent excision and experienced recurrence of perineal warts since the age of 28 years. Clinical examination showed oval hyperchromi...

In recent studies (Tarshis, 1952, 1953) it was found that a modified human blood medium was more satisfactory for the cultivation of tubercle bacilli than several other blood media previously described (Tarshis and Frisch, 1951; Frisch and Tarshis, 1951). Since comparison of this medium under routine diagnostic condition was somewhat limited, a more thorough evaluation was felt desirable. In th...

We compared Mycobacterium tuberculosis sputum culture recovery and contamination rates between Lowenstein-Jensen medium (LJ) containing the following decontaminants and LJ alone: (i) PANTA (n = 299), (ii) Selectatab-MB (n = 299), and (iii) penicillin G (n = 234). The contamination rate for LJ alone was approximately 31%, versus 5.0% for PANTA-containing, 2% for Selectatab-containing, and 9% for...

Methods This prospective study was done at NMC, Nellore from July 2008-December 2009. Samples received at microbiology lab for acid fast staining were included in this study. Smears were stained by Ziehl-Neelsen’s technique. Samples were cultured on Lowenstein-Jensen media after processing by modified Petroff’s method and incubated according to CLSI guidelines. Identification of Mycobaterium tu...