many recent studies have shown that glycosylation patterns of agaricus bisporus are similar to those of mammalians, so that this organism is a good candidate for the expression of glycosylated pharmaceutical protein. to achieve constant interested gene expression in all cells of the organism, proper promoter isolation is necessary. to isolate this promoter, pcr with specific primers was perform...

background anaerobic-inducible promoters are alternatives of chemical-inducible promoters for expression of recombinant proteins especially in conditions where chemical induction is not possible or anaerobic conditions are preferable. the nirb promoter is the promoter of the first gene of nir operon in escherichia coli, which encodes nadh-dependent nitrite reductase. this promoter is naturally ...

The Covid-19 disaster has impacted the learning process, not only for normal children but also with special needs. They have same right to get a quality education. This community service is addressed as an outdoor model and applies health protocol at LPK Kaizen Wireless Center (KNC). KNC institution that provides education training services method used consultation, support facilities following...

Background: Epigenetic modifications, such as methylation can occur in multiple myeloma. SMG1is an important gene involved in cell growth which defect in methylation of its promoter leads to reduction of cell apoptosis and uncontrolled proliferation. In this study, we identified the methylation status of the SMG1 gene promoter in patients with multiple myeloma. Methods: Methylation status of S...

Dalam persaingan global dan era revolusi industri 4.0 dewasa ini, sumber daya manusia (SDM) memegang peranan sangat vital dalam perusahaan agar mampu bersaing. Penelitian ini bertujuan untuk mengetahui pengaruh komitmen organisasi memediasi motivasi kerja disiplin terhadap kinerja pegawai pada LPK Monarch Bali. Jenis penelitian yang digunakan adalah kuantitatif. Sampel survei 115 karyawan di Me...

There are two different co-expression systems including bicistronic; dual-vector or two-promoter to express two different genes simultaneously and also to study protein-protein interactions. Bicistronic system has disadvantages e.g. compared with two-promoter system. In this paper, a simple method based on spliced overlap extension by polymerase chain reaction (SOE-PCR) technique was demonstrat...

objective: in an attempt to develop safer and more effective gene therapy approaches as a realistic treatment for various forms of cancer, researchers are increasingly using tumor-specific promoters (tsp) to drive the expression of the gene of interest and eradicate cancer cells. in this study, for the first time we introduce the oct-4 promoter as a cancer-specific promoter with a high efficacy...