Epstein-Barr virus association in nonproducer human lymphoblastoid cell lines can be demonstrated by the presence of the virus genome (nucleic acid hybridization studies) or by the detection of the virus-coded complement-fixing antigen (complement fixation and/or anti-complement immunofluorescent test). This paper describes an enzyme immunoassay for the detection of Epstein-Barr virus complemen...

Nemaline myopathy (NM) is a congenital typically characterized by skeletal muscle weakness and the presence of abnormal thread- or rod-like structures (nemaline bodies) in myofibres. Pathogenic variants alpha actin gene, ACTA1, cause approximately 25% all NM cases. We generated two induced pluripotent stem cell lines from lymphoblastoid cells 4-month-old female with severe harbouring dominant v...

In this study we examined HLA-F expression in normal cells and cell lines, with a particular focus on identifying cells that express surface protein. While HLA-F protein was expressed in a number of diverse tissues and cell lines, including bladder, skin, and liver cell lines, no surface expression could be detected in the majority of them. However, surface expression was observed on EBV-transf...

Research on voltage-dependent anion channel (VDAC) has accelerated as evidence grows of its importance in mitochondrial function and in apoptosis. In this report, we studied the effect of VDAC contributed to the apoptosis of the cell lines carrying mitochondrial DNA A4263G mutation. We established lymphoblastoid cell lines derived from 3 symptomatic and 1 asymptomatic hypertension individuals i...

OBJECTIVES Epstein Barr virus (EBV)-transformed lymphoblastoid cell lines (LCLs) are a useful biological resource, however, genomic variations can happen during the generation and immortalization processes of LCLs. The purpose of this study was to identify genomic variations in LCL DNA compared with matched blood DNA using short tandem repeats (STRs) analysis. METHODS We analyzed 15 STRs with...

In vitro infection of human B lymphocytes with Epstein-Barr virus (EBV) results in establishment of B lymphoblastoid cell lines that reflect normal B cell phenotypes. In this study we have investigated whether immature B cells from fetal bone marrow and liver can serve as targets for EBV. The fetal bone marrow cells were readily transformed by EBV. Among the resulting cell lines, five were surf...