objective: hippocampal insults have been observed in multiple sclerosis (ms) patients. fibroblast growth factor-2 (fgf2) induces neurogenesis in the hippocampus and enhances the proliferation, migration and differentiation of oligodendrocyte progenitor cells (opcs). in the current study, we have investigated the effect of fgf2 on the processes of gliotoxin induced demyelination and subsequent r...

We used a rapid and simple protocol using lysolecithin for mapping HS sites in vivo. The protocol is based on partial digestion with DNase I of exponentially growing cells following permeabilization by short treatment with lysolecithin. Using this protocol, we analyzed the chromatin structure of the region surrounding two overlapping elements, an origin of bidirectional DNA replication and the ...

The ability of lipid vesicles of simple composition (lecithin, lysolecithin, and stearylamine) to induce cells of various types to fuse has been investigated. One in every three or four cells in monolayer cultures can be induced to fuse with a vesicle dose of about 100 per cell. At such dosages and for exposures of 15 min to 1 h, vesicles have essentially no effect on cell viability. Under anae...

The guanylate cyclase activity [GTP pyrophosphate-lyase (cyclizing), EC 4.6.1.2] in membrane preparations from 3T3 mouse fibroblasts is stimulated approximately 5-fold by lysolecithin at concentrations of 100 mug/ml and above.

Multiple sclerosis (MS) is one of the most autoimmune neurological and inflammatory disease in worldwide. Demyelination and disturbance of action potential conductance are regarded as main signs of MS disease. Hesperetin (Hst) is one of the flavonoid that have neuroprotective properties. The present study attempts to evaluate the effects of hesperetin or its nanoparticle on myelin repair and gl...

The plasma membrane fraction of rat liver was isolated and incubated with labeled lysophosphatides in the presence of cofactors; the acylation of lysolecithin to lecithin by the fraction was compared to that of the rough and smooth microsomes. The purity of the isolated fractions was ascertained by enzyme markers and electron microscopy, and the maximal contamination of the plasma membrane frac...

This paper is concerned with the nature of the hemolytic material which occurs in certain tumors, and particularly with the question whether it dif fers from the hemolytic material found in many normal tissues. The latter, at least in one of its forms, has been shown to have the characteristics of soaps and of lysolecithin-like substances more or less loosely combined with inhibitory material i...

The report of Oakley, Warrack, and Clarke (1947) on the toxins of the Clostridium novyi (oedematiens) group and of Clostridium hemolyticum indicates the complexity of this topic and presents the pertinent historical data. It is known that the toxins C. novyi and C. hemolyticum involve a lecithinase catalyzing the hydrolysis of lecithin to phosphorylcholine and a diglyceride. In view of the exis...