Objective. To study the antibacterial activity of lysostaphin against staphylococci various species, as well its effect on viability Vero cells. Materials and Methods. Lysostaphin was obtained by genetic engineering. Purification protein carried out SP-sepharose, purity determined electrophoresis in PAGE Lamley. The susceptibility to 9 species 175 strains studied. Identification performed MALDI...

Staphylococcus aureus infections exert a tremendous burden on the health-care system, and the threat of drug-resistant strains continues to grow. The bacteriolytic enzyme lysostaphin is a potent antistaphylococcal agent with proven efficacy against both drug-sensitive and drug-resistant strains; however, the enzyme's own bacterial origins cause undesirable immunogenicity and pose a barrier to c...

The effect of incubation temperatures of 41, 37, and 4 degrees C on phagocytosis was investigated using human neutrophils and [3H]thymidine-labeled staphylococci. Depressed phagocytosis was observed at 41 and 4 degrees C. At 41 degrees C diminished staphylococcal uptake resulted from decreased attachment of bacteria to leukocytes; the inhibitory effect at 4 degrees C was secondary both to decre...

Department of Microbiology, Melaka Manipal Medical College (Manipal Campus) Manipal University, Manipal, Karnataka 576104, India Department of Pharmaceutical Biotechnology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka 576104, India Department of Pharmaceutical Chemistry, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka 5761...

Bacteriophage lytic enzymes have recently attracted considerable interest as novel antimicrobials against Gram-positive bacteria. In this work, antimicrobial activity in milk of HydH5 [a virion-associated peptidoglycan hydrolase (VAPGH) encoded by the Staphylococcus aureus bacteriophage vB_SauS-phiIPLA88], and three different fusion proteins created between HydH5 and lysostaphin has been assess...

Some strains of Staphylococcus epidermidis and Micrococcus sp. produce nucleases. However, thermal stability was shown to be unique to the nucleases of S. aureus. In addition, two micromethods for susceptibility testing to lysostaphin were more precise and convenient than anaerobic glucose fermentation in distinguishing between the genera Staphylococcus and Micrococcus.

A derivative of Staphylococcus simulans biovar staphylolyticus cured of all five plasmids present in the wild-type organism was developed, and the characteristics of extracellular protein production by this plasmidless strain were compared to those of the wild type. Although staphylolytic endopeptidase (lysostaphin) and beta-lactamase are known to be plasmid encoded, analysis of this cured stra...