In order to determine whether human granulocyte-macrophage colony-stimulating factor (GM-CSF) can enhance phagocytosis. neutrophils were combined with Staphylococcus aureus (S aureus). and both the number of bacteria per neutrophil and the percent of neutrophils phagocytizing were assessed in the absence and presence of GM-CSF. Exposure to GM-CSF did not enable neutrophils to ingest unopsonized...

In order to determine whether human granulocyte-macrophage colony-stimulating factor (GM-CSF) can enhance phagocytosis, neutrophils were combined with Staphylococcus aureus (S aureus), and both the number of bacteria per neutrophil and the percent of neutrophils phagocytizing were assessed in the absence and presence of GM-CSF. Exposure to GM-CSF did not enable neutrophils to ingest unopsonized...

the present study aimed at investigating the beneficial effects of co-administering granulocyte colony–stimulating factor (g-csf) and stem cell factor (scf) in a model of chronic liver injury induced by thioacetamide (taa). biochemical and histopathology- cal examinations were performed on serum and liver specimens. at the end of the treatment period, the rats were anesthetized with ether, seru...

OBJECTIVE To characterize and evaluate hematopoietic colony-stimulating factor (CSF) use, including cost implications, in US academic health centers. DESIGN An observational study of patients who received granulocyte colony-stimulating factor (G-CSF) or granulocyte-macrophage colony-stimulating factor (GM-CSF) from September 1 to October 15, 1993. SETTING Thirty academic health centers in t...

Transcription of early growth response gene-1 (Egr-1) is required for macrophage differentiation. Since granulocyte-macrophage colony-stimulating factor (GM-CSF) induces macrophage proliferation as well as the transcription of Egr-1 mRNA, we characterized the induction of Egr-1 protein in murine peritoneal macrophages following treatment with GM-CSF. As determined by Western blot analysis, the ...

The cytokine Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) regulates proliferation, differentiation, and apoptosis during myelopoiesis and erythropoiesis. Structure-function relationships of GM-CSF interactions with its receptor (GM-R), the biochemistry of GM-R signal transduction, and GM-CSF action in vivo are relatively well understood. Much less is known, however, about GM-R func...

Human and mouse granulocyte-macrophage-colony-stimulating factors (hGM-CSF and mGM-CSF, respectively), isolated from Escherichia coli cells expressing the corresponding human and mouse genes, have been characterized. The observed properties of the proteins have been compared with those properties which can be deduced from the DNA sequence alone and the published properties of natural GM-CSFs. T...

Canine granulocyte-macrophage colony-stimulating factor (caGM-CSF) was cloned and expressed to allow further investigation of GM-CSF in a large animal model. The cDNA is 850 base pairs (bp) long and encodes a peptide of 144 amino acids. The nucleotide and amino acid sequence homology between caGM-CSF and human GM-CSF (hGM-CSF) is 80% and 70%, respectively. A mammalian expression vector pCMV/CAG...

X-irradiation of purified primary cultures of mouse bone marrow stroma or permanent cloned marrow stromal cell lines in plateau phase decreases production of macrophage progenitor cell-specific colony-stimulating factor to a plateau minimum of 40% of control levels after doses of 50 to 500 Gy delivered at 2 Gy/min. After 50 Gy there is increased bioavailability of another growth factor(s) that ...