Dyes for measuring lysosome pH (pHlys) cannot be propagated in cells longitudinal or vivo studies. We report a new genetically encoded ratiometric pHlys biosensor, pHLARE (pH Lysosomal Activity REporter), which we use to show decreased with inhibiting mTORC1 (mammalian target of rapamycin complex 1) activity and cancer different tissues genetic signatures.

Adenoviruses are used extensively to deliver genes into mammalian cells, particularly where there is a requirement for high-level expression of transgene products in cultured cells, or for use as recombinant viral vaccines or in gene therapy. In spite of their usefulness, the construction of adenoviral vectors (AdV) is a cumbersome and lengthy process that is not readily amenable to the generat...

background: phic31 integrase system provides a new platform in various felid of research, mainly in gene therapy and creation of transgenic animals. this system enables integration of exogenous dna into preferred locations in mammalian genomes, which results in robust, long-term expression of the integrated transgene.objectives: identification of a novel pseudo attp site.materials and methods: ...

Back Cover: In article 2200377, Cyrille Boyer and co-workers show the antimicrobial polymers as a promising approach to combat multidrugresistant pathogens. A library of 31 amphiphilic ternary containing different cationic groups, including primary amine, guanidine, sulfonium is prepared investigate impact groups on activity cytotoxicity against mammalian cells.

Chromosomes isolated from mouse leukemia L1210 cells were taken up by mouse macrophages, HeLa cells, and rat embryo fibroblasts following simple exposure in vitro. The process, which resembles pinocytosis or phagocytosis, was traced by autoradiography of chromosomes prelabeled with thymidine-H(3), and by staining techniques and phase contrast microscopy. During the first six hours, the uptake o...

The physiologic function of the immune system is defense against infectious microbes and internal tumour cells, Therefore, need to have precise modulatory mechanisms to maintain the body homeostasis. The mammalian cellular CD200 (OX2)/CD200R interaction is one of such modulatory mechanisms in which myeloid and lymphoid cells are regulated. CD200 and CD200R molecules are membrane proteins that t...