نتایج جستجو برای: meca gene

تعداد نتایج: 1142208  

Journal: :MANAS journal of engineering 2022

Milk and its products can be frequently contaminated with enterotoxigenic methicillin-resistant S. aureus, in such a case, it causes various diseases, especially staphylococcal food poisoning. In the present study, 100 sample materials (50 goat milk, 25 cream, cheese) were collected from 65 livestock farms Erzurum. All samples analyzed tested selectively according to EN ISO 6888-1 procedure sta...

Journal: :Antimicrobial agents and chemotherapy 2014
Feng-Jui Chen Chen-Her Wang Ching-Yi Chen Yu-Chieh Hsu Kaun-Ting Wang

The most prevalent community-associated methicillin-resistant Staphylococcus aureus (C-MRSA) strains in Taiwan, sequence type 59 (ST59) clones, carry staphylococcal cassette chromosome mec (SCCmec) type V and, to a lesser extent, type IV. These strains show wide variation in sensitivity to oxacillin, but the reasons for this variation are unknown. Here we compared the sequences of the mecA gene...

2011
Laura García-Álvarez Matthew TG Holden Heather Lindsay Cerian R Webb Derek FJ Brown Martin D Curran Enid Walpole Karen Brooks Derek J Pickard Christopher Teale Julian Parkhill Stephen D Bentley Giles F Edwards E Kirsty Girvan Angela M Kearns Bruno Pichon Robert LR Hill Anders Rhod Larsen Robert L Skov Sharon J Peacock Duncan J Maskell Mark A Holmes

BACKGROUND Animals can act as a reservoir and source for the emergence of novel meticillin-resistant Staphylococcus aureus (MRSA) clones in human beings. Here, we report the discovery of a strain of S aureus (LGA251) isolated from bulk milk that was phenotypically resistant to meticillin but tested negative for the mecA gene and a preliminary investigation of the extent to which such strains ar...

Background: Methicillin resistant Staphylococcus aureus (MRSA) has been emerged as a nosocomial and community acquired pathogen worldwide. There are many challenges for laboratory detection of MRSA. The aim of this study was to compare different phenotypic methods with PCR based method as a gold standard for detection of mecA gene. <b...

Journal: :Journal of clinical microbiology 2013
V Anil Kumar Katherin Steffy Maitrayee Chatterjee Madhan Sugumar Kavitha R Dinesh Anand Manoharan Shamsul Karim Raja Biswas

Reports of oxacillin-susceptible mecA-positive Staphylococcus aureus strains are on the rise. Because of their susceptibility to oxacillin and cefoxitin, it is very difficult to detect them by using routine phenotypic methods. We describe two such isolates that were detected by chromogenic medium and confirmed by characterization of the mecA gene element.

Journal: :Journal of clinical microbiology 2005
Yuki Katayama D Ashley Robinson Mark C Enright Henry F Chambers

The staphylococcal methicillin resistance determinant, mecA, resides on a mobile genetic element, staphylococcus chromosomal cassette mec (SCCmec). The distribution of SCCmec in nature is limited to relatively few clonal complexes of related methicillin-resistant Staphylococcus aureus (MRSA). We have previously reported that some genetic backgrounds are restrictive of mecA and penicillin-bindin...

ژورنال: پیاورد سلامت 2018
امینی, کیومرث, محمد جانی, فاطمه,

Background and Aim: Staphylococcus aureus produces many virulence factors, including toxins, immune-modulatory factors, and exoenzymes. The study was performed to determine virulence and resistance-related factors etA, etB, tst, mecA and femA in the S. aureus isolated from clinical samples using Multiplex PCR. Materials and Methods: In this cross - sectional study, a total 60 S. aureus were co...

ایمنی, نازیلا, فرح‌بخش, حمید, قیامی راد, مهدی, نامی, یوسف,

Background and Objective: Due to the emergence and development of antimicrobial resistance in coagulase-negative Staphylococci (CoNS), which is mainly a normal flora of the skin surface and mucous membrane of humans, and the limitation of therapeutic options, this study was aimed to investigate the antibiotic resistance pattern in CoNS strains isolated from clinical specimens. Materials and Me...

2016
Hossein Koupahi Sahar Honarmand Jahromy Mohammad Rahbar

BACKGROUND Methicillin resistant Staphylococcus aureus (MRSA) has been emerged as a nosocomial and community acquired pathogen worldwide. There are many challenges for laboratory detection of MRSA. The aim of this study was to compare different phenotypic methods with PCR based method as a gold standard for detection of mecA gene. METHODS A total of 220 clinical isolates of S. aureus which we...

2013
Olugbenga Adekunle Olowe Olayinka Oluwatoyin Kukoyi Samuel Sunday Taiwo Olusola Ojurongbe Oluyinka Oladele Opaleye Oloyede Samuel Bolaji Abiodun Adebimpe Adegoke Olufunmilola Bamidele Makanjuola David Olusoga Ogbolu Oyebode Terry Alli

INTRODUCTION The characteristics and antimicrobial resistance profiles of Staphylococcus aureus differs according to geographical regions and in relation to antibiotic usage. The aim of this study was to determine the biochemical characteristics of the prevalent S. aureus from Ekiti State, Nigeria, and to evaluate three commonly used disk diffusion methods (cefoxitin, oxacillin, and methicillin...

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