Metabolite profiling is the multiparallel relative quantification of a mixture of compounds or compound classes using chromatography and universal detection technologies (GC-MS, LC-MS). In this respect it is an extension of classical single-target methods from which it can be distinguished by its broader view on profiling major biochemical events. This broader scope of analysis outweighs the di...

Azonafide (2-[2'-(dimethylamino)ethyl]-1,2-dihydro-3H-dibenz[de, h]isoquinoline-1,3-dione) is the parent of a new series of anthracene-containing antitumor agents. Its structure is based on amonafide but lacks a primary amine and has an anthracene chromophore rather than a naphthalene chromophore. Using a rat liver cytosol incubation and HPLC/MS detection, we have identified four metabolites re...

We have previously determined that 6-amino-2-benzothiazolesulfonamide (aminozolamide) significantly lowers IOP in rabbits and, more importantly, in ocular hypertensive human subjects. Results from in vitro experiments established that both the inhibitory activity of aminozolamide against carbonic anhydrase B as well as the penetration rate across excised rabbit corneas were equivalent to ethoxz...

A metabolite profiling study of the antibiotic producing bacterium Streptomyces coelicolor A3(2) has been performed. The aim of this study was to monitor intracellular metabolite pool changes occurring as strains of S. coelicolor react to nutrient depletion with metabolic re-modeling, so-called metabolic switching, and transition from growth to secondary metabolite production phase. Two differe...

Drug metabolism and pharmacokinetics (DMPK) studies require the identifi cation of drug metabolites usually by mass spectrometry (MS), and in later stages of drug development, confi rmation of metabolite structures by NMR. To achieve this, purifi cation of metabolites may be required. In this Application Note, purifi cation of bupropion drug metabolites was achieved using Agilent Automated Puri...

In metabolomics the identity and role of low mass molecules called metabolites that are produced in cell metabolic processes are investigated. These make them valuable indicators of the phenotype of a biological system. The ‘Metabolite Space’ is the total chemical universe of metabolites present in all compartments and in all states from any organism. These molecules exhibit common features tha...

The success of metabolomics studies depends upon the “fitness” of each biological sample used for analysis: it is critical that metabolite levels reported for a biological sample represent an accurate snapshot of the studied organism’s metabolite profile at time of sample collection. Numerous factors may compromise metabolite sample fitness, including chemical and biological factors which inter...

Tanaproget is a first-in-class nonsteroidal progesterone receptor agonist that is being investigated for use in contraception. A major in vitro and in vivo metabolite of tanaproget formed in humans was initially characterized as a glucuronide of tanaproget. However, whether the glucuronide was linked to the nitrogen or sulfur of the benzoxazine-2-thione group in tanaproget could not be determin...