We examined the chromatin structure of the rat metallothionein I gene, both in uninduced cells and in cells induced by heavy metals or dexamethasone, using hypersensitivity to DNase I as an assay. The metallothionein I gene of the H4IIE rat hepatoma cell line, expressed at basal level, has a single DNase I-hypersensitive site. This site maps between putative hormone and basal level control sequ...

Nanospray and collisionally-induced dissociation are used to evaluate the presence and absence of interstrand co-chelation of zinc ions in dimers of metallothionein. As was reported in a previous publication from this laboratory, co-chelation stabilizes the dimer to collisional activation, and facilitates asymmetrical zinc ion transfers during fragmentation. In the case of metallothionein, dime...

Abstract Herein, we report a new simple and easy-to-use approach for the characterization of protein oligomerization based on fluorescence resonance energy transfer (FRET) capillary electrophoresis with LED-induced detection. The FRET pair consisted quantum dots (QDs) used as an emission tunable donor (emission wavelength 450 nm) cyanine dye (Cy3), providing optimal optical properties acceptor....

Abstract. Kadim MK, Herawati FY, Arfiati D, Hertik AMS. 2022. Metallothionein level in the larva of Cheumatopsyche sp. and relationship between heavy metal concentration Bone River, Gorontalo, Indonesia. Biodiversitas 23: 5942-5950. This study aims to confirm that larvae contain a metal-binding protein, metallothionein, their body correlate it with concentrations larval tissues sediments River....

  Background and Objective: In this study, we explored expression rate, some biomarkers affecting the prognosis of the breast carcinoma, and the relationship between these markers and clinicopathologic features of the disease as well as the relationship between each of these markers through a tissue array technique. Materials and Methods: This study was an observational and cross-sectional st...

BACKGROUND In many tumors including ovarian cancer, cell proliferation and apoptosis are important in pathogenesis and there are many alterations in most of the genes related to the cell cycle. This study was designed to evaluate immunohistochemistry with apoptotic-antiapoptotic proteins (p53, p21, bax, and bcl-2), c-kit, telomerase, and metallothionein as a diagnostic aid in typing of benign, ...

The hepatic concentration of a low molecular weight, metal-binding protein in lto 4-day-old rats is 20 times that in 70-day-old adults. Biochemical purifications were performed to characterize this metal-binding protein in the liver of lto 4-day-old rats. Gel filtration of hepatic cytosol on Sephadex G-75 demonstrated that the major Zn-containing fraction of newborns had a relative elution volu...

Metallothionein (MT) is a ubiquitous protein with a low molecular weight of 6-7 kDa weight and it was first identified in the kidney cortex of equines as a cadmium (Cd)-binding protein responsible for the natural accumulation of Cd in the tissue. The mammalian MT contains 61 to 68 amino acid residues, in which 18 to 23 cysteine residues are present. The expression of MT starts by binding of met...

AIMS Reduced insulin sensitivity following alcohol intake plays a role in alcohol-induced organ damage although its precise mechanism is undefined. This study was designed to examine the effect of cardiac overexpression of the antioxidant metallothionein on alcohol-induced cardiac contractile dysfunction and post-receptor insulin signaling. METHODS FVB and metallothionein mice were fed a 4% a...