Non-coding RNAs (ncRNAs) recently were discovered to outnumber their protein-coding counterparts, yet their diverse functions are still poorly understood. Here we report on a method for the intracellular Single-molecule High-Resolution Localization and Counting (iSHiRLoC) of microRNAs (miRNAs), a conserved, ubiquitous class of regulatory ncRNAs that controls the expression of over 60% of all ma...

We present the first results obtained with a new instrument designed and built to study DNA-protein interactions at the single molecule level. This microscope combines optical tweezers with scanning probe microscopy and allows us to locate DNA-binding proteins on a single suspended DNA molecule. A single DNA molecule is stretched taut using the optical tweezers, while a probe is scanned along t...

Treatment of CuF(2) with 2 equiv of 3{5}-[pyrid-2-yl]pyrazole (Hpz(Py)), 3{5}-phenylpyrazole (Hpz(Ph)) or 3{5}-[4-fluorophenyl]pyrazole (Hpz(PhF)) in MeOH, followed by evaporation to dryness and recrystallisation of the solid residues, allows solvated crystals of [{Cu(micro-pz(Py))(pz(Py))}(2)] (1), [{Cu(micro-pz(Ph))(2)}(4)] (2) and [Cu(4)F(2)(micro(4)-F)(micro-pz(PhF))(5)(Hpz(PhF))(4)] (3) to...

This paper reports on the state-of-the-art of technologies for the design and fabrication of MEMS micro-mirrors. We discuss the major design issues, considerations, calculations and restrictions of micro mirrors. The materials employed for the reflective surfaces are described along with their properties. The materials used for the static and dynamic MEMS micro structures as well as the differe...

the compressibility factor of nonassociated chain molecules composed of hard convex core yukawa segments was derived with saft-vr and an extension of the barker-henderson perturbation theory for convex bodies. the temperature-dependent chain and dispersion compressibility factors were derived using the yukawa potential. the effects of temperature, packing fraction, and segment number on the com...

background:the polysialylated neural cell adhesion molecule (psa-ncam) is expressed in developing brain. fetal brain damage is caused by different conditions such as seizure and hypoxia. the present study was designed to investigate the effect of maternal seizures on the number of psa-ncam positive cells in pup's hippocampus. methods: female wistar rats were divided into four groups: (a) k...

Abstract This paper presents a probe-based force-controlled nanoindentation method to fabricate ordered micro/nanostructures. Both the experimental and finite element simulation approaches are employed investigate influence of interval between adjacent indentations rotation angle probe on formed The non-contacting part indenter sample material height pile-up two competing factors determine dept...

The dielectric properties of pure PVC and filled composites were performed to examine characterize the structure polymer composites. At room temperature values relative permittivity, loss factor, ac conductivities calculated as a function frequency it was found that incorporation both micro nano PbO CuO with various wt% matrix resulted in enhanced properties. electrical response micro- or nanoc...

Reactions between two novel hexatopic carboxylate ligands and copper salts give rise to two isostructural metal-organic frameworks (MOFs) with a (3,24)-connected network topology containing both micro- and mesocavities with sizes of up to 3 nm. Both frameworks retain their porosity after guest-molecule removal, leading to high surface areas. Constructing MOFs containing mesocavities with microw...