نتایج جستجو برای: micromass culture
تعداد نتایج: 277586 فیلتر نتایج به سال:
H and C NMR spectra were recorded with Bruker DPX-400, AVANCE-400 and DRX-500. The numbering of the protons relate to the numbering chosen in Fig. 1. High resolution mass spectra were obtained with a Micromass LCT via loop-mode injection from a Waters (Alliance 2695) HPLC system. Alternatively a Micromass Q-TOF in combination with a Waters Aquity Ultraperformance LC system was employed. Ionizat...
In humans, loss of SHOX gene function is responsible for the mesomelic short stature characteristic of Turner syndrome, Leri-Weill dyschondrosteosis, and Langer dysplasia. In a mouse model of SHOX deficiency, Prrx1-Cre-driven limb-specific deletion of the paralogous gene Shox2 results in severe rhizomelia. In this study, we show that Col2a1-Cre-driven deletion of Shox2 in developing chondrocyte...
INTRODUCTION The control of differentiation of mesenchymal stromal/stem cells (MSCs) is crucial for tissue engineering strategies employing MSCs. The purpose of this study was to investigate whether the transcriptional co-factor Yes-associated protein (YAP) regulates chondrogenic differentiation of MSCs. METHODS Expression of total YAP, its paralogue transcriptional co-activator with PDZ-bind...
INTRODUCTION Notochordal cells (NCs) are influential in development of the intervertebral disc (IVD) and species that retain NCs do not degenerate. IVD repair using bone marrow derived mesenchymal stem cells (MSCs) is an attractive approach and the harsh microenvironment of the IVD suggests pre-differentiation is a necessary first step. The goal of this study was to use soluble factors from NCs...
selon les pédagogues, la culture étant un élément très important dans le processus de lapprentissage de langue étrangère et la maîtrise de la compétence linguistique requiert celle de la compétence culturelle. la littérature dun pays est comme un miroir qui reflète bon nombre daspects culturels, cest pourquoi avec le choix dun texte littéraire convenable, lenseignant peut assurer à la fois lapp...
Mesenchymal stem cells (MSC) are a potential alternative source of differentiated chondrocytes for cartilage tissue regeneration and repair of osteoarthritic (OA) joints. We investigated the effects of pentosan polysulfate (PPS) and polysulfated glycosaminoglycan (PSGAG) on chondrogenesis of canine bone marrow-derived mesenchymal stem cells (cBMSC) in alginate and micromass cultures (MMC). Chon...
PHOSPHO1 is a bone-specific phosphatase implicated in the initiation of inorganic phosphate generation for matrix mineralization. The control of mineralization is attributed to the actions of tissue-nonspecific alkaline phosphatase (TNAP). However, matrix vesicles (MVs) containing apatite crystals are present in patients with hypophosphatasia as well as TNAP null (Akp2(-/-)) mice. It is therefo...
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