Newborn Screening for the diagnosis of Congenital Hypothyroidism (CH) was first started in Bangladesh 1999 by Immunoradiometric assay (IRMA). Dissociation enhanced lanthanide fluorescent immunoassay (DELFIA) 1986 due to its superior sensitivity, speed, and non-radioactive methodology. Auto DELFIA 1235 automatic system introduced In vitro Division National Institute Nuclear Medicine & Allied...

PURPOSE With use of the Wong-Kilbourne derivative Chang conjunctival cell line, this study compared in vitro the ocular toxicity of three topical intraocular pressure (IOP)-lowering agents: travoprost 0.004% containing 0.015% benzalkonium chloride (BAK), travoprost Z 0.004%, a new formulation without BAK, and latanoprost 0.005% containing 0.02% BAK. METHODS Neutral red, Alamar blue, YOPRO-1, ...

BACKGROUND Determinations of certain forms of prostate-specific antigen (PSA) have been shown to increase the specificity for prostate cancer (PCa). One such variant, proteolytically active PSA, is a potentially useful tumor marker, but it is not specifically recognized by antibodies. Using phage display libraries, we previously identified a "family" of peptides that bind specifically to active...

A new, rapid method for the detection of human immunodeficiency virus type 1 (HIV-1) antibody by time-resolved fluoroimmunoassay (TR-FIA) was developed. In this assay format, microtitration strips were coated with a recombinant fusion protein, and the same protein was labeled with europium and added into the wells simultaneously with the test specimens. The recombinant fusion protein contained ...

OBJECTIVES To study the effects of GSTM1, GSTT1 gene polymorphisms, and organism antioxidant capacity and related indicators such as antioxidant capacity per unit of albumin (AC/ALB) on chronic obstructive pulmonary disease (COPD). METHODS Using polymerase chain reaction technology, GSTM1 and GSTT1 gene polymorphisms were detected in 33 COPD patients and 33 healthy people. The total antioxida...

BACKGROUND Microalbuminuria is an established early marker of diabetic nephropathy and an important cardiovascular risk factor in diabetes and hypertension. We aimed to develop a rapid point-of-care assay for the measurement of urine albumin. METHODS The competitive homogeneous assay used an albumin-specific monoclonal antibody labeled with a stable fluorescent europium chelate as donor and a...

The estimation of ammonia in the blood is a difficult matter not. only because ammonia is present in very minute quantities, but also because it is rapidly split off from some labile components of shed blood. The removal of the ammonia by means of an air current is subject to a source of error in as much as the amount of ammonia recovered may vary with the rapid&y of the aeration and the temper...

A Laboratory Branch Task Force of the National Communicable Disease Center has proposed a standardized complement fixation procedure (LBCF) and an adaptation of this to microtitration techniques (MT) as uniform methods for performing complement fixation (CF) tests. A common procedure should make CF results from one laboratory more comparable to another. In addition, it would be preferable if th...

Numerous non-isotopic techniques have been developed in the last decade to detect and quantitate hybridization products (1). Since the polymerase chain reaction (PCR) was introduced this technique has become increasingly important for the diagnosis of bacterial and viral infections. The need for simple methods allowing quantitative evaluation of PCR products has resulted in the development of a...