Five ruminally cannulated steers (initial BW = 375 ± 34 kg; ADG = 1.16 ± 0.13 kg) were used in a 5 x 5 Latin square design experiment to determine the effects of increasing urea level on intake, nutrient digestion, nitrogen balance and ruminal kinetics. Isocaloric (NEm = 2.11 Mcal/kg; NEg = 1.35 Mcal/kg) and isonitrogenous (2.24% N) steam-flaked corn-based diets with urea concentrations of 0.0,...

Microbial protein synthesis of steers (liveweight 286±9 kg) was compared with diets of ad libitum winter grass and fodder beet with 1 kg DM of lucerne silage. The experimental design was a four-by-two treatment comparison (experiment 1 and 2), using 11 day individual pen trials with total faecal and urinary collection. The trials assessed in vivo digestibility of diets, and diurnal variations i...

One primiparous and 3 multiparous lactating Holstein cows fitted with ruminal and duodenal cannulas were used in a 4 x 4 Latin square design to determine the efficacy of adding urea to a corn silage-based diet on ruminal fermentation and microbial protein synthesis. Dietary treatments were 0, 0.3, 0.6, and 0.9% urea in diet dry matter (DM); urea was manually top dressed and incorporated into th...

The effects of supplying increasing ruminal doses of exogenous polysaccharide-degrading enzymes (EPDE) on rumen fermentation and nutrient digestion were studied using eight ruminally cannulated heifers, four of which were also duodenally cannulated, in a replicated Latin square. The heifers were fed a diet of 85.5% rolled barley grain and 14% barley silage (DM basis), and once daily they were g...

The objective of this study was to investigate the effect of carbohydrate source and cottonseed meal level in the concentrate on feed intake, nutrient digestibility, rumen fermentation and microbial protein synthesis in swamp buffaloes. Four, 4-yr old rumen fistulated swamp buffaloes were randomly assigned to receive four dietary treatments according to a 2×2 factorial arrangement in a 4×4 Lati...

_Treating the wastewater with high level of urea and ammonia-nitrogen is one of the problems faced by urea fertilizer plants in Indonesia. The alternative treatment being studied is the one which uses Fenton oxidation process which is continued with the use of Pseudomonas fluorescens bacteria. This study is conducted with the concentration of ammonia-nitrogen of 2500 ppm, 2000 ppm, and 1500 ppm...

The study was conducted to assess the effect of milk production, parity, stage of lactation, season and individual milk components themselves on milk urea nitrogen (MUN) concentration and other milk components of 3,219 Holstein dairy cows in Korean dairy farms. The MUN concentrations in Korean dairy cows were estimated to 16.68±5.87 mg/dl. Milk yield was negatively correlated with fat and prote...

Four crossbred (75% Holstein Friesian) lactating dairy cows, with an average live weight of 418±5 kg and 36±10 d in milk were randomly assigned according to a 2×2 factorial arrangement in a 4×4 Latin square design to evaluate the effects of cassava hay (CH) and rice bran oil (RBO) on feed intake, nutrient digestibility, ruminal fermentation, milk yield, and milk composition. Factor A was non-su...

Our objectives were to determine effects of grain processing on splanchnic (gut tissues and liver) N metabolism and whole-body N balance by growing steers and to ascertain the relative contributions of ruminal and intestinal tissues to net absorption and utilization of N-containing nutrients. Seven beef steers (348 kg initial BW), surgically implanted with appropriate catheters, were fed diets ...

Mixed model analysis of data from 32 studies (122 diets) was used to evaluate the precision and accuracy of the omasal sampling technique for quantifying ruminal-N metabolism and to assess the relationships between nonammonia-N flow at the omasal canal and milk protein yield. Data were derived from experiments in cattle fed North American diets (n=36) based on alfalfa silage, corn silage, and c...