The cellular response to DNA damage that has been most extensively studied is the SOS response of Escherichia coli. Analyses of the SOS response have led to new insights into the transcriptional and post-translational regulation of processes that increase cell survival after DNA damage as well as insights into DNA-damage-induced mutagenesis, i.e., SOS mutagenesis. SOS mutagenesis requires the r...

One of the main impediments for obtaining DNA sequences from ancient human skeletons is the presence of contaminating modern human DNA molecules in many fossil samples and laboratory reagents. However, DNA fragments isolated from ancient specimens show a characteristic DNA damage pattern caused by miscoding lesions that differs from present day DNA sequences. Here, we develop a framework for ev...

Polychlorosubtilin (PCS) inhibits the growth of Escherichia coli. The antibiotic affected neither respiration nor glycolysis while the synthesis of nucleic acids and proteins were feebly hindered. Formation of aminoacyl-tRNA, peptide bonds and translocation from A to P sites of ribosomes were insignificantly influenced by the drug. The antibiotic exerted its effect(s) on ribosomes by interferin...

BACKGROUND Birth weight mortality statistics are important for examining trends and monitoring the outcomes of neonatal care. AIM To determine the effects of errors in the registered birth weight on birth weight specific mortality. METHODS All twins born in England and Wales during 1993-95 comprise the denominator population. For those twins that died, the Office for National Statistics (ON...

Hypothesis / aims of study By law, all surgical procedures carried out at Danish hospitals are reported to the National Patient Registry (LPR) according to the ICD10 coding system. The following year a report is published. The codes for very different procedures are very much alike for which reason there is a potential risk of miscoding. Furthermore, there are no options to code for the complex...

The mrili) lliulr;t/ini"s. monomi lli>llmlra/inr, 1,1-dimethylhydrazinc, and 1,2-dimethyHiydrazinc, are known carcinogens but only weak mutagens in the Ames test. Chemical oxidation of these compounds by potassium fcrricyanide greatly enhanced their mutagenicity to an Escherichia coli atla mutant and converted them into inducers of the adaptive response of /•'. col! to alkylation damage. Knzy...