one of the important methods of plant propagation in large scale so as genetic manipulation and artificial seed production is somatic embryogenesis technique. the obtained results from this methods related to different factors including condition of culture medium. therefore, the aim of this study conduction were determining the role of molybdenum during carrot petiole somatic embryogenesis. at...

Interferon is a protein secreted by animal cells, when stimulated by certain agents, which has the ability to induce in homologous cells a resistance to replication of a wide variety of viruses (1, 2). Moreover, preparations of mouse interferon, both crude and partially purified, have been shown to inhibit the growth of mouse L cells (3) and mouse lymphoid leukemia cells (4) in suspension cultu...

Isolation of Mycoplasma genitalium from clinical specimens remains difficult. We describe a modified culture system based on Vero cells grown in medium 199 with 2% foetal bovine serum (FBS). The culture system was evaluated using early passage M. genitalium strains M6271 and M6311 with growth monitoring by quantitative TaqMan PCR. Eleven endocervical swabs and one male urethral swab positive by...

The large scale in vitro cultivation method of Fairlamb et al (1985) was modified to contain human plasma-supplemented medium in HEPES buffer. After 4 days with no change of medium nor agitation of the culture flask, 27- to 50-fold increase in the starting parasitemia of trophozoites were obtained.

background: in the field of mammalian embryo culture, the putative influence of autocrine/ paracrine factor(s), produce by the embryos itself, is under investigation. a smaller medium drop can prevent dilution of this factor(s). objective: the objective of this study was to examine the effect of culture medium volume on in vitro development of mouse 2-cell embryos. materials and methods: the em...

the germination power of orchids (orchidaceae family) seems to betoo weak due lack of albumen. the study carried out with various treatments including pollination time and ga3 for breaking dormancy and increasing seed germination of orchids. the effect of pollination time (8 periods from january to august) and gibberellic acid (0, 500, 1000 and 1500 mg l-1) were studied on germination of phalae...