Antibodies not only play a major role in clinical diagnostics and biopharmaceutical analysis but also are class of drugs that regularly used to treat numerous diseases. The identification antibody-epitope binding sites is then great interest many emerging medical bioanalytical applications, particularly design monoclonal antibodies (mAb) mimics taking advantage amino acid residues involved the ...

Rabies virus antigen-specific human monoclonal antibodies (MAbs) that recognized either viral glycoprotein, ribonucleoprotein, or matrix proteins were generated. Only glycoprotein-specific MAb neutralized a variety of rabies viruses and protected laboratory rodents against lethal rabies virus infection. The determinant recognized by this MAb does not appear to reside in previously defined antig...

Two monoclonal antibodies (mAb) within cluster M4 of the myeloid section of the Second International Swine CD Workshop, C4 (No. 144) and PM18-7 (No. 192), showed reactivity with thymocytes and among cells of myelomonocytic origin with mature macrophages but not with monocytes and granulocytes. Both mAb recognize a protein showing two bands of 205 kDa and 130 kDa under both reducing and non-redu...

Abstract Although human antibodies elicited by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid (N) protein are profoundly boosted upon infection, little is known about function of N-reactive antibodies. Herein, we isolate and profile a panel 32 N protein-specific monoclonal (mAbs) from quick recovery disease-19 (COVID-19) convalescent patient who has dominant antib...

Ultraviolet (UV)-induced DNA damage and skin inflammation are well-known mechanisms of carcinogenesis. However, the extent to which contributes UV-induced carcinogenesis is unknown. The xeroderma pigmentosum group A (XPA) model mice (Xpa-KO mice) deficient in repair systems, making them susceptible cancer. We previously reported that blood levels CXCL1, a neutrophil attractant chemokine, increa...

Monoclonal antibodies (Mabs) were produced against the early Polish strains of infectious bursal disease virus (IBDV) isolated from subclinical cases of Gumboro disease between 1978 and 1980. Splenocytes from immunized mice were fused to myeloma cells and hybridomas producing antibodies against homologous and different pathotype of IBDVs were selected by indirect ELISA, immunoperoxidase, ELISA-...

Rabbit anti-idiotypic antibodies (alpha Id Ab) were prepared against five murine monoclonal antibodies (mAb) specific for the rabies virus glycoprotein. Four of the mAb were directed against three known, type-specific, neutralizing sites on the glycoprotein, and the other mAb was directed against a topographically uncharacterized, nonneutralizing epitope. An absence of significant cross-reactiv...

Bacillus anthracis is the causative agent of anthrax, and the tripartite anthrax toxin is an essential element of its pathogenesis. Edema factor (EF), a potent adenylyl cyclase, is one of the toxin components. In this work, anti-EF monoclonal antibodies (MAb) were produced following immunization of mice, and four of the antibodies were fully characterized. MAb 3F2 has an affinity of 388 pM, was...

Monoclonal antibodies (mAb or moAb) are monospecific antibodies which are made from identical immune cells that are all clones of a unique parent cell. Monoclonal antibodies have monovalent affinity, in that they bind to the same epitope 1. Monoclonal antibodies (mAbs) are currently used for many diagnostic and therapeutic applications. Advances in the development of mAb-producing cell lines ar...

Immunoreactive prolactin (ir-PRL) in rat brain has been consistently documented. However, the identity of this ir-PRL is controversial. Ir-PRL is defined by its ability to bind to PRL antibodies. All previous studies of brain ir-PRL have used polyclonal antibodies, at least one of which apparently crossreacts with a portion of the proopiomelanocortin molecule. To begin to define the epitopes co...