objective: vitrification has been shown as one of the most effective methods of cryopreservation for mammalian embryos. however, there is no consensus which stage of embryonic development is the most appropriate for vitrification with subsequent maximal development after thawing. this study was carried out to explore and compare the effect(s) of vitrification on mouse 2-cell, 4-cell, 8-cell, mo...

In mammals, active demethylation of sperm pronuclear DNA shortly after fertilisation is thought to be important for reprogramming subsequent embryonic development [1–3]. A further passive loss of methylation has been observed as DNA replicates between 2-cell and morula stages, with somatic cell levels being re-established at or after the blastocyst stage when differentiated lineages are first f...

The objective was to evaluate development of cloned embryos constructed with serum-starved versus fed ovarian granulosa cells. Fusion of somatic cells with cytoplasts (50.7 and 50.7%; SEM=5.5), development of cloned embryos to 8-16-cell (59.4 and 62.6%; SEM=6.4), and compact morula/blastocyst-stages (24.9 and 26.5%; SEM=8.5) was similar for serum-starved and fed groups. More heifers were confir...

fusion is a developmental anomaly defined as the :union: of two normally separated tooth buds or the partial splitting of one tooth bud into two buds. depending on the stage of development, fusion may be either complete or incomplete. the significance of this particular case was that this fusion occurred in a posterior permanent mandibular tooth, while such a manifestation is more reported in m...

BACKGROUND The ability to cryopreserve mammalian embryos has become an integral part of assisted reproduction, both in human and veterinary medicine. Despite differences in the size and physiological characteristics of embryos from various species, the embryos have been frozen by either of two procedures: slow freezing or vitrification. The aim of our study was to compare the effect of slow fre...

Bovine oocytes were matured, fertilized, and cultured FCM 199 with serum and co-culture) in vitro (IVMFC) with addition, during different phases of the procedure, of antioxidants: superoxide dismutase (SOD) and reduced glutathione (GSH). The addition of SOD (1,500 or 3,000 IU/ml) did not improve proportions of oocytes undergoing cleavage or the development of embryos to morula and blastocyst st...

The inclusion of specific amino acids in conventional culture media has been shown to enhance mammalian embryo development in vitro. Amino acids have been shown to confer their benefits to the preimplantation embryo in a number of different ways. However, their ability to buffer intracellular pH (pHi) has not been investigated. Thus, the aim of this study was to determine if amino acids regulat...

in this study, mutant forms of bacillus thuringiensis spp. israelensis (h14) were produced. these mutants were identified when the cells were cultured on chloramphenicol plates and stained with crystal violet. protoplasts of the mutants were isolated by enzymatic digestion (lysozyme) of the cell walls at the presence of an osmotic stabilizer. the protoplasts were induced to fuse to each other i...

fusion is an anomaly manifested in both deciduous and permanent dentitions. fu- sion of dental tissues in the primary dentition is of clinical significance owing to the challenges in treatment of the affected teeth and aberrations encountered in development and eruption of their successors. triple tooth refers to the :union: of three separate tooth entities. it can occur by fusion, germination,...