We have developed a quantitative method for methylation analysis of the p16 gene based on real-time methylation-specific PCR (MSP). Real-time MSP is sensitive enough to detect down to 10 genome equivalents of the methylated p16 sequence. Application of real-time MSP to DNA from tumor-derived cell lines revealed complete concordance with conventional MSP analysis. Quantitative data generated by ...

OBJECT This study aimed to investigate the diagnostic value of placenta-derived macrophage-stimulating protein α-chain (MSP-α) before the 20th week of gestation for the early diagnosis of preeclampsia (PE). METHODS AND MATERIALS Two parts of this nested case-control study were simultaneously executed, and 1500 pregnant women were recruited. A total of 124 pregnant women were included in the p...

In this paper, we introduce a so-called ‘Multistage graph Simple Path’ (MSP) problem and show that the Hamilton Circuit (HC) problem can be polynomially reducible to the MSP problem. To solve the MSP problem, we propose a polynomial algorithm and prove its NP-completeness. Our result implies NP=P.

the number of PCR products that exhibited methylated-and unmethylated-p16 of two representative GC samples as determined using the 83bp-MSP assay. The lower panel compares the detection of methylated-p16 in 61 gastric tissue samples using the 83bp-MSP and sMSP assays. The table indicated that the results of the 83bp-MSP correlated with those of sMSP significantly (p<0.04).

In this study of human polymorphonuclear leukocytes (PMNs), pretreatment with Treponema denticola major outer sheath protein (Msp) inhibited formyl-methionyl-leucyl-phenylalanine (fMLP)-induced chemotaxis, phagocytosis of immunoglobulin G-coated microspheres, fMLP-stimulated calcium transients, and actin assembly. Msp neither altered oxidative responses to phorbol myristate or fMLP nor induced ...

We have added support for the Sound Description Interchange Format to the Max/MSP environment. We briefly introduce SDIF and Max/MSP, describe how SDIF data is represented in MSP and how to write programs to manupulate SDIF data, and demonstrate example applications.

Multiple Statistical Prototypes (MSP) is a modification of a standard minimum distance classification scheme that generates multiple prototypes per class using a modified greedy heuristic. Empirical comparison of MSP with other well-known learning algorithms shows MSP to be a robust algorithm that uses a very simple premise to produce good generalization and achieve parsimonious hypothesis repr...

Millettiae speciosae Champ. Leguminosae (MSC), is a well-known Chinese herb traditionally used as food material and medicine for enhancing physical strength. Our preliminary study found that the aqueous extract of this herb (MSE) had an anti-fatigue effect. In this paper, we further separated MSE into total polysaccharides (MSP) and supernatant (MSS) by alcohol precipitation, and explored which...

Leishmania chagasi is the causative agent of visceral leishmaniasis in South America. The most abundant glycoprotein on the surface of L. chagasi promastigotes is the glycosylphosphatidylinositol (GPI) anchored protease MSP (major surface protease), also called GP63. MSP is encoded by more than 18 tandem MSP genes on a single chromosome. MSP genes are classified according to unique sequences at...

The Plasmodium falciparum malaria parasite is the causative agent of malaria tropica. Merozoites, one of the extracellular developmental stages of this parasite, expose at their surface the merozoite surface protein-1 complex (MSP-1), which results from the proteolytic processing of a 190-200 kDa precursor. MSP-1 is highly immunogenic in humans and numerous studies suggest that this protein is ...