BACKGROUND The Bacillus subtilis spore has long been used to display antigens and enzymes. Spore display can be accomplished by a recombinant and a non-recombinant approach, with the latter proved more efficient than the recombinant one. We used the non-recombinant approach to independently adsorb two thermophilic enzymes, GH10-XA, an endo-1,4-β-xylanase (EC 3.2.1.8) from Alicyclobacillus acido...

One approach for facilitating protein function prediction is to classify proteins into functional families. Recent studies on the classification of G-protein coupled receptors and other proteins suggest that a statistical learning method, Support vector machines (SVM), may be potentially useful for protein classification into functional families. In this work, SVM is applied and tested on the c...

Many turtles exhibit temperature-dependent sex determination. We have examined the hypothesis that incubation temperature causes a differential expression of steroidogenic enzymes in embryonic turtles. The activities of three steroidogenic enzymes were studied histochemically in turtle (Trachemys scripta) embryos at different developmental stages: sexually undifferentiated (Stage 15), different...

Abstract Within cells, the close spatial arrangement of cascade enzymes facilitates channeling intermediates and enhances reaction efficiency. Reconfigurable DNA nanostructures, owing to their structural controllability precise addressability, are promising tools for mimicking such processes. In this study, a 3D origami scaffold, with dynamic shape transformation from its open boat form closed ...

Multi-copper oxidases (MCOs) are a small group of enzymes that oxidize their substrate with the concomitant reduction of dioxygen to two water molecules. Generally, multi-copper oxidases are promiscuous with regards to their reducing substrates and are capable of performing various functions in different species. To date, three multi-copper oxidases have been detected in humans--ceruloplasmin, ...

raspberry waste, obtained from two cultivars, meeker (erm) and willamette (erw) was subjected to evaluation antioxidants and antitumour activities as a potential source of phenolics. some phenolic acids and flavonoids were identified and quantified by hplc. antioxidant activity was tested by measuring ability to scavenge dpph• and •oh by esr spectroscopy. ic50dpph• varied from 0.67 for erm to 0...

thyrotoxicosis is a condition in which tissues are stimulated by increased secretion of thyroid hormone. the most common cause is diffuse toxic goiter and toxic multi-nodular goiter. for more reviews on this disease, the effects of hyperthyroidism on liver enzyme levels were studied. a total of 30 adult male wistar rats weighing about 190 g were purchased from the pasteur institute of iran. in ...

Abstract Delftia sp. K82 is a soil bacterium capable of utilizing monocyclic aromatic hydrocarbons, including aniline, as its sole carbon and nitrogen source. In this study, the genome analysis was completed data (6117 protein-coding genes 62 RNA genes) were utilized for proteomic transcriptomic cultured in aniline culture medium. Using these multi-omics approaches (genomics, transcriptomics, p...

the genus fusarium is a species rich genus. different media are required to study themorphological characters and classify different species, a time consuming technique. in this study pecticzymograms were used to identify species and form species of fusarium isolates. fusarium isolates (318) wereobtained from different areas and hosts in isfahan province, iran. isolates were identified to speci...