is more sensitive but requires two steps [two rounds of amplification (PCR LDR) are needed]. The costs for setup are larger (PCR primers and three labeled oligonucleotides are needed per SNP) with respect to PCR/RFLP and allele-specific PCR but are similar for the TaqMan assay. The main advantage consists in the inherent multiplexing capability that can increase throughput, which decreases cost...

Self-incompatibility (SI) is a genetic mechanism in many flowering plants by which generative reproduction prevented. The self-incompatibility caused the functions of cell controlled genes called S or genes. results decreased pollination and ultimately yield loss. In apple (Malus domestica L.), multi-allelic S-locus. Approaches S-glycoprotein profiles allele-specific PCR methods using gene for ...

Objective(s): The population in Iran is a genetic admixture of the ancestral Aryan and other populations neighboring Iran. Different ethnic groups in Iran show wide regional distributions for many human leukocyte antigen (HLA) alleles. Therefore, it is necessary and sensible to study the differences in HLA allele distribution in different area. We studied the HLA class I and II allele frequenci...

This study was conducted to assess the possible association between polymorphisms of transcription factor 7-like 2 (TCF7L2) gene and obese Iraqi adults. DNA obtained from 158 subjects 142 controls. Two specific PCR fragments were designed incorporate two highly frequent single nucleotide polymorphism (SNP)s within TCF7L2, rs11196208 rs7908486. Both amplified loci genotyped by PCR-single-strand ...