نتایج جستجو برای: mutant

تعداد نتایج: 129581  

Journal: :Methods in molecular biology 2014
Janine N Copp Paulina Hanson-Manful David F Ackerley Wayne M Patrick

Any single-enzyme directed evolution strategy has two fundamental requirements: the need to efficiently introduce variation into a gene of interest and the need to create an effective library from those variants. Generation of a maximally diverse gene library is particularly important when employing nontargeted mutagenesis strategies such as error-prone PCR (epPCR), which seek to explore very l...

2012
Felix Sahm David Capper Matthias Preusser Jochen Meyer Albrecht Stenzinger Felix Lasitschka Anna-Sophie Berghoff Antje Habel Marion Schneider Andreas Kulozik Ioannis Anagnostopoulos Leonhard Müllauer Gunhild Mechtersheimer Andreas von Deimling

1Department of Neuropathology, Institute of Pathology, Ruprecht-Karls-Universität Heidelberg, Heidelberg, Germany; 2Clinical Cooperation Unit Neuropathology, German Cancer Research Center (DKFZ), Heidelberg, Germany; 3Department of Medicine I and Comprehensive Cancer Center, Medical University of Vienna, Vienna, Austria; 4Department of General Pathology, Institute of Pathology, Ruprecht-Karls-U...

Journal: :jundishapur journal of microbiology 0
samia siddique department of chemistry, government college university lahore, lahore, pakistan quratulain syed food and biotechnology research center, pcsir laboratories complex, ferozpur lahore, lahore, pakistan ahmad adnan department of chemistry, government college university lahore, lahore, pakistan fahim ashraf qureshi office of research, innovation and commercialization, comsats institute of information and technology, islamabad, pakistan; office of research, innovation and commercialization, comsats institute of information and technology, islamabad, pakistan. tel: +92-3334101261, fax: +92-519247008

conclusions: the hereditary stability analysis of the uv mentioning 45 minutes revealed the uv exposure time for mutants and 3 represented the colony taken from the plate irradiated for 45 minutes mutant showed that the production of avermectin b1b remained constant and no reverse mutation occurred after 15 generations. results: avermectin b1b-hyper-producing mutant, produced using these three ...

Journal: :پژوهش های علوم دامی ایران 0
حامد خراتی کوپایی محمد رضا محمد آبادی سعید انصاری مهیاری علی اسماعیلی زاده کشکوئیه علیرضا ترنگ مهدی نیکبختی

. the results of multiple qtl mapping design in recent years indicated quantitative trait loci for milk fat percentage and milk yield traits identified in bta14. dgat1 gene coding diacylglycerol-acyltransferase enzyme, has a main role in the triglyceride synthesis and finally fat milk synthesis. a transition mutation in this gene results substitution of guanine by adenine in the gene causing su...

Journal: :Molecular Systems Biology 2009
Quan Zhong Nicolas Simonis Qian-Ru Li Benoit Charloteaux Fabien Heuze Niels Klitgord Stanley Tam Haiyuan Yu Kavitha Venkatesan Danny Mou Venus Swearingen Muhammed A Yildirim Han Yan Amélie Dricot David Szeto Chenwei Lin Tong Hao Changyu Fan Stuart Milstein Denis Dupuy Robert Brasseur David E Hill Michael E Cusick Marc Vidal

Cellular functions are mediated through complex systems of macromolecules and metabolites linked through biochemical and physical interactions, represented in interactome models as 'nodes' and 'edges', respectively. Better understanding of genotype-to-phenotype relationships in human disease will require modeling of how disease-causing mutations affect systems or interactome properties. Here we...

Journal: :Protein engineering, design & selection : PEDS 2010
Majid Masso Iosif I Vaisman

Utilizing cutting-edge supervised classification and regression algorithms, three web-based tools have been developed for predicting stability changes upon single residue substitutions in proteins with known native structures. Trained models classify independent mutant test sets with accuracies ranging from 87 to 94%. Attributes representing each mutant protein are based on a computational muta...

Journal: :The Journal of biological chemistry 1988
D S Hwang J M Kaguni

Highly purified preparations of dnaA46 protein have permitted its biochemical characterization in comparison with the activities of wild type dnaA protein. We have determined that dnaA46 protein was reduced in its ability to bind to DNA fragments containing oriC. This mutant protein was also defective in binding ATP and was inactive for replication of oriC-containing plasmids in purified enzyme...

Water deficient stress is one of the major limiting factors of wheat production in arid and semiarid areas of the world such as Iran. Under water deficient stress photosynthesis is limited and remobilization of assimilates stored in stems is more important to grain filling. In order to molecular analysis of fructan remobilization in wheat stem (penultimate) under terminal water deficit stress, ...

Journal: :Phytopathology research 2022

Abstract Small GTPases of the Rab family play important roles in membrane trafficking and autophagy. Previous studies have shown that small GTPase Sec4 is involved vegetative growth, protein secretion, pathogenicity fungal phytopathogens. In this study, BcSec4, an ortholog yeast Sec4p protein, was characterized Botrytis cinerea . The ΔBcSEC4 mutant produces many abnormal conidiophores; it thus ...

Journal: :Translational lung cancer research 2023

Background: The tumor immune microenvironment influences evolution in non-small cell lung cancer (NSCLC). Yet, the prognostic value of programmed death-ligand 1 (PD-L1) epidermal growth factor receptor (EGFR)-mutant NSCLC remains controversial. Additionally, studies Filipinos with EGFR-mutant remain unexplored to this day.

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