background :  young chickens are more susceptible to salmonella colonization than older ones that have developed resistance with age as native microflora become established.   methods :  in this study, two groups of fertile eggs were inoculated with 20 cfu of hila or parent strains of s . enteritidis . presence and number of salmonella cells inside the homogenized egg contents were determined on...

The effect of Ultra-Violet (UV) irradiation and acridine orange dye on L-glutamic acid producing Bacillus subtilis B. megaterium was investigated. selected mutant strains wild types were immobilized used for production. exposed to UV treated with respectively. survived found reduce as the exposure time concentration mutagens increased in this study. Four mutants two Mutant strain (MAIR4) yielde...

The DDK syndrome is an early embryonic lethal phenotype observed in crosses between females of the DDK inbred mouse strain and many non-DDK males. Lethality results from an incompatibility between a maternal DDK factor and a non-DDK paternal gene, both of which have been mapped to the Ovum mutant (Om) locus on mouse chromosome 11. Here we define a 465-kb candidate interval for the paternal gene...

Hemochromatosis is caused by mutations in HFE, a protein that competes with transferrin (TF) for binding to the transferrin receptor (TFR1). We developed mutant mouse strains to gain insight into the role of the Hfe/Tfr1 complex in regulating iron homeostasis. We introduced mutations into a ubiquitously expressed Tfr1 transgene or the endogenous Tfr1 locus to promote or prevent the Hfe/ Tfr1 in...

Previous studies have indicated that the yellow pigments (xanthomonadins) produced by phytopathogenic Xanthomonas bacteria are unimportant during pathogenesis but may be important for protection against photobiological damage. We used a Xanthomonas campestris pv. campestris parent strain, single-site transposon insertion mutant strains, and chromosomally restored mutant strains to define the bi...

Global warming has been considered to accelerate the expansion of cyanobacterial blooms, which are frequently composed bloom-forming genera, Microcystis and Pseudanabaena, in freshwater ecosystems worldwide. Nonetheless, impact changes due toxin production or lack thereof on growth co-existing strains, both arising from increasing temperature, remained unknown date. We conducted competition exp...

Fibronectins (FNs) are multifunctional high molecular weight glycoproteins present in the blood plasma and in the ECMs of tissues. The FN primary transcript undergoes alternative splicing in three regions generating up to 20 main different variants in humans. However, the precise role of the FN isoforms is poorly understood. One of the alternatively spliced exons is the extra domain A (EDA) or ...

The 2 auxiliary subunits ( 2 -1 and 2 -2) of voltage-sensitive calcium channels are thought to be the site of action of pregabalin (Lyrica), a drug that has been shown to be anxiolytic in clinical trials for generalized anxiety disorder. Pregabalin and the chemically related drug gabapentin have similar binding and pharmacology profiles, demonstrating high-affinity, in vitro binding to both 2 -...

An extracellular alkaline lipase from Pseudomonas aeruginosa mutant has been purified to homogeneity using acetone precipitation followed by anion exchange and gel filtration chromatography and resulted in 27-fold purification with 19.6% final recovery. SDS-PAGE study suggested that the purified lipase has an apparent molecular mass of 67 kDa. The optimum temperature and pH for the purified lip...

We determined the genotypes of > 200 offspring that are survivors of matings between female reciprocal F1 hybrids (between the DDK and C57BL/6J inbred mouse strains) and C57BL/6J males at markers linked to the Ovum mutant (Om) locus on chromosome 11. In contrast to the expectations of our previous genetic model to explain the "DDK syndrome, " the genotypes of these offspring do not reflect pref...