Phagosome biogenesis, the process by which macrophages neutralize ingested pathogens and initiate antigen presentation, has entered the field of cellular mycobacteriology research largely owing to the discovery 30 years ago that phagosomes harboring mycobacteria are refractory to fusion with lysosomes. In the past decade, the use of molecular genetics and biology in different model systems to s...

Objective(s) Rapidly growing mycobacteria (RGM) are capable of producing diseases in humans. Since mycobacteria vary in their susceptibility, precise identification is critical for adoption of correct drug therapy. The main aim of this study was molecular identification and evaluation of antimicrobial susceptibility pattern of Iranian clinically isolated Myocbacterium fortuitum. Materials and...

Abstract DNA is basically an intracellular molecule that stores genetic information and carries instructions for growth reproduction in all cellular organisms. However, some bacteria, has additional roles outside the cells as extracellular (eDNA), which essential component of biofilm formation hence antibiotic tolerance. Mycobacteria include life-threating human pathogens, most are slow growers...

"Non-tuberculous mycobacteria" (NTM) is a general term for pathogenic mycobacteria other than Mycobacterium tuberculosis and leprae. Non-tuberculous are widely present in the natural environment, including soil water, with over 150 reported species. abscessus rare among mycobacteria, there few reports of extrapulmonary lesions due to abscessus. We describe case osteomyelitis an adult woman's le...

It is generally accepted that cellular, and not humoral immunity, plays the crucial role in defense against intracellular bacteria. However, accumulating data indicate the importance of humoral immunity for the defense against a number of intracellular bacteria, including mycobacteria. We have investigated the role of secretory IgA, the main isotype found in mucosal tissues, in protection again...

By crossed immunoelectrophoresis and Western blotting (immunoblotting), it was shown that Borrelia burgdorferi expresses the 60-kilodalton Common Antigen (CA) that is cross-reactive with an equivalent antigen in a wide range of remotely related bacteria. B. burgdorferi CA is strongly immunogenic. A B. burgdorferi genomic library was constructed by using a plasmid cloning system. Escherichia col...

A markedly improved immunofluorescent technic employing FITC conjugated IgG antibody prepared from lepromatous serum is described as a means of specific identification of M. leprae. An additional immunologic identification method for M. leprae is presented as a micro-complement fixation technic employing antigen rather than antibody dilution. Studies with these technics suggest that M. leprae s...

The potentially pathogenic Non-Tuberculosis Mycobacteria (NTM) are emerging nowadays which result in pulmonary and non-pulmonary infections in human. This group of bacteria consists of at least 200 different species. While the pulmonary disease is the most common form of NTM infections, NTM can cause diffused infections as well as extrapulmonary infections in every organ, such as bone marrow, s...