Coronary heart disease (CHD) and myocardial infarction (MI) account for ≈1 in 7 deaths in the United States, with an estimated 750 000 incidences of MI in the United States each year. Although modern medical therapies have reduced the number of coronary heart disease–related deaths over the past 30 years, there is still much room for improvement in coronary heart disease and MI treatment. As ca...

The production of a functional cardiac tissue to be transplanted in the injured area of the infarcted myocardium represents a challenge for regenerative medicine. Most cell-based grafts are unviable because of inadequate perfusion; therefore, prevascularization might be a suitable approach for myocardial tissue engineering. To this aim, cells with a differentiation potential towards vascular an...

This protocol provides methods for the preparation of an injectable extracellular matrix (ECM) gel for myocardial tissue engineering applications. Briefly, decellularized tissue is lyophilized, milled, enzymatically digested, and then brought to physiological pH. The lyophilization removes all water content from the tissue, resulting in dry ECM that can be ground into a fine powder with a sma...

Future perspectives Heart disease is a leading cause of morbidity and mortality worldwide. Myocardial infarction leads to permanent loss of cardiac tissue and ultimately heart failure. However, current therapies could only stall the progression of the disease. Thus, new therapies are needed to regenerate damaged hearts to overcome poor prognosis of patients with heart failure. The shortage of h...

Adult and pluripotent stem cells represent a ready supply of cellular raw materials that can be used to generate the functionally mature cells needed to replace damaged or diseased heart tissue. However, the use of stem cells for cardiac regenerative therapies is limited by the low efficiency by which stem cells are differentiated in vitro to cardiac lineages as well as the inability to effecti...

at present, iran has been known one of the up-warding countries in the world in regenerative medicine using stem cells therapy. in fact, the outcomes of some clinical trials on stem cell therapy of myocardial infarction, vitiligo, decompensated cirrhosis, and osteoarthritis narrate the feasibility of stem cell-based therapy for treatment of human diseases 1,2. however, in a similar manner with ...

BACKGROUND Skeletal myoblast transplantation seems a promising approach for the repair of myocardial infarction (MI). However, the low engraftment efficacy and impaired angiogenic ability limit the clinical efficiency of the myoblasts. Gene engineering with angiogenic growth factors promotes angiogenesis and enhances engraftment of transplanted skeletal myoblasts, leading to improved infarction...

It is of value to determine the amount of viable myocardial tissue in patients suffering from chronic coronary artery disease and ventricular dysfunction. Having the capability of evaluating both myocardial perfusion and function, simultaneously, myocardial scanning by ECG-Gated is an appropriate method for this purpose. The aim of this study was to compare the results of myocardial perfu...

OBJECTIVE Embryonic stem cell-derived cardiomyocytes are a useful source for cell transplantation into the heart, as well as for tissue engineering of the extracardiac vascular system. The present study was designed to investigate the survival and contractile function of embryonic stem cell-derived cardiomyocytes around large blood vessels to assess the feasibility of their ectopic use for futu...

Measuring In Vivo Myocardial Substrate Competition Using Hyperpolarized 13C Magnetic Resonance Jessica A.M. Bastiaansen, Matthew E. Merritt, and Arnaud Comment Laboratory of Functional and Metabolic Imaging, EPFL, Lausanne, Switzerland, Advanced Imaging Research Center,Department of Radiology,Molecular Biophysics,Biomedical Engineering, UTSW Medical Center, Texas, United States, Institute of Ph...