BACKGROUND Measurements of CFTR function in rectal biopsies ex vivo have been used for diagnosis and prognosis of Cystic Fibrosis (CF) disease. Here, we aimed to evaluate this procedure regarding: i) viability of the rectal specimens obtained by biopsy forceps for ex vivo bioelectrical and biochemical laboratory analyses; and ii) overall assessment (comfort, invasiveness, pain, sedation require...

The effect of NaCl plus 3% chitosan on the systolic blood pressure of spontaneously hypertensive rats (SHR) were evaluated and compared with NaCl plus KCl (NaCl, 49.36% + KCl 49.36%) and chitosan or NaCl treatment alone. In SHR, administration of NaCl plus chitosan (44 mM Na/day) for two months significantly decreased the systolic blood pressure greater than of NaCl plus KCl and NaCl alone. NaC...

Downlo PIM family of serine threonine protein kinases plays an important role in regulating both the growth and rmation of malignant cells. However, in a cell line–dependent manner, overexpression of PIM1 can t cell and tumor growth. In 22Rv1 human prostate cells, but not in Du145 or RWPE-2, PIM1 oversion was associated with marked increases in cellular senescence, as shown by changes in the le...

acrjourna h levels of the critical p53 inhibitor Mdm4 is common in tumors that retain a wild-type p53 allele, ting that Mdm4 overexpression is an important mechanism for p53 inactivation during tumorigenesis. t this hypothesis in vivo, we generated transgenic mice with widespread expression of Mdm4. Two ndent lines of transgenic mice, Mdm4 and Mdm4, developed spontaneous tumors, the most prevf ...

English teachers are frequently asked to help colleagues prepare presentations for internationalconferences. Sometimes this assistance takes the form of a language course or tutorials focusingon the spoken language. Contact time is short but the participants are highly motivated. Whattype of pronunciation work will provide the greatest payoff in terms of successfully beingunders...

in this study, we assessed the relative contribution of organic and inorganic solutes to osmotic adjustment (oa) in three almond rootstocks subjected to four levels of soil salinity. the results showed that leaf water and osmotic potentials were affected by salinity in gf677 and bitter almond, but less so in gn15, suggesting a higher selectivity for k+ and ca2+ against na+ in this latter rootst...

für Naturforschung in cooperation with the Max Planck Society for the Advancement of Science under a Creative Commons Attribution 4.0 International License. Dieses Werk wurde im Jahr 2013 vom Verlag Zeitschrift für Naturforschung in Zusammenarbeit mit der Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. digitalisiert und unter folgender Lizenz veröffentlicht: Creative Commons Namen...

BACKGROUND The laboratory diagnosis of hereditary spherocytosis commonly relies on NaCl-based or glycerol-based red cell osmotic fragility tests; more recently, an assay directly targeting the hereditary spherocytosis molecular defect (eosin-5'-maleimide-binding test) has been proposed. None of the available tests identifies all cases of hereditary spherocytosis. DESIGN AND METHODS We compare...

Salinity is one of the major factors that limits geographical distribution of plants and adversely affects crop productivity and quality. We report here high-level expression of betaine aldehyde dehydrogenase (BADH) in cultured cells, roots, and leaves of carrot (Daucus carota) via plastid genetic engineering. Homoplasmic transgenic plants exhibiting high levels of salt tolerance were regenerat...

7S globulins were extracted from common bean (Phaseolus vulgaris L.) seeds and characterized. SDS-PAGE showed major bands corresponding to the phaseolin subunits (43-53 kDa). An amino acid analysis indicated that, in spite of the limited amounts of sulphur amino acids and tryptophan, the globulins contained very high levels of essential amino acids. The protein solubility profiles of native and...