Patients with hypercholesterolaemia are often treated with the antimicrobial agent neomycin. Such treatment is potentially dangerous, however, as it may favour the emergence of multiresistant, R-factor-carrying, enteric bacteria among the intestinal flora. In 11 out of 14 patients who had received neomycin for three months to eight years most of the faecal coliforms were resistant to at least f...

Immunoassays greatly contribute to veterinary drug residue analysis. However, there are few reports on detecting neomycin residues by immunoassay. Here, a rapid and sensitive chemiluminescent enzyme immunoassay (CLIEA) was successfully developed for neomycin residue analysis. CLIEA demonstrated good cross-reactivity for neomycin, and the IC50 value was 2.4 ng/mL in buffer. The average recovery ...

To better define a minimal but optimal dose of oral neomycin to suppress ammonia production by bowel flora, several dosage regimens were examined in normal healthy volunteers. Fecal urease activity was quantitatively determined and was used as an indirect measure of intrinsic ammonia production by bowel flora. Large doses of neomycin were found to exert inhibition of fecal urease for many days....

Try. N m n a o M a s ~ N~OC~,NO ~,crmN of several commonly employed antibiotics is now well established, x Respiratory arrest in humans has been attributed to intraperitoneal installation of neomycin, 2,8 kanamycin, 4 and streptomycin, ~,e to intraplueral neomycin, 7 and to streptomycin e and intravenous kanamyein, s In several isolated instances, neostigmine-resistant curarization occurred in ...

The presence of a number of minor components was demonstrated in commercial samples of neomycin by column chromatography on a weakly acidic ionexchange resin (Amberlite CG-50). Some of these components were isolated by ionexclusion chromatography and identified. The identification was based on chromatographic and nmr data and on mass spectrometry of volatile derivatives. The sequence of elution...

The neo (neomycin-resistance) gene of transposon Tn5 encodes the enzyme neomycin phosphotransferase II (EC 2.7.1.95), which confers resistance to various aminoglycoside antibiotics, including kanamycin and G418. The gene is widely used as a selectable marker in the transformation of organisms as diverse as bacteria, yeast, plants, and animals. We found a mutation that involves a glutamic to asp...

Neomycin and lincomycin are widely used in the treatment of infections illnesses. However, irrational inappropriate antibiotics contributes to threaten human health seriously. We developed a rapid multiple detection strategy for trace amounts neomycin (NEO) (LIN) milk using Surface-Enhanced Raman Spectroscopy (SERS)-based lateral flow assay (LFA). The method two SERS immunoprobes (a probe NEO L...

Effects of neomycin were studied on serum cholesterol and fecal steroids in hypercholesterolemic patients during a short treatment period (4 wk) and a long treatment period (16 mo), using small (1.5 g/d) and large (up to 6 g/d) doses alone and in combination with cholestyramine. In the short-term low-dose study the decrease in serum cholesterol by 21% was associated with a proportionate increas...

Aminoglycosides (including neomycin B and tobramycin) exhibit poor uptake by eukaryotic cell lines. When the amines of these natural products are converted into guanidine groups, their cellular uptake is dramatically enhanced. We have synthesized BODIPY-containing aminoglycosides and guanidinoglycosides to evaluate their cellular uptake properties. Fluorescence activated cell sorting (FACS) and...

The present study aimed to investigate the prevalence of Campylobacter (C) and its clinicopathological changes in broilers Sharkia province, About 50 diarrheic cloacal swabs were collected for bacteriological examination. Out exami-ned 12 (24%) positive [C. coli 4 C. jejuni 8]. Isolated was sensitive neomycin genta-ycin. 45 healthy one day-old received 5 mg pefloxacin/kg bwt days proved that fr...