egg drop syndrome (eds) is caused by a hemagglutinating adenovirus which has become a major cause of lost egg production and sever economic losses in breeder and layer chicken flocks throughout the world. a pcr assay was optimized for detection of eds virus in inoculated allantoic fluids (afs) of duck and chicken emberyonated eggs. two strains of eds viruses were propagated in allantoic cavity ...

The role of proteins in biomineralization and the mechanism of eggshell formation are not well understood. We have isolated and purified the major protein, ansocalcin from goose eggshell matrix. The amino acid sequence study indicates that ansocalcin is homologous to the chicken eggshell protein, ovocleidin 17, and C-type lectins. Ansocalcin nucleates polycrystalline aggregates of calcite cryst...

Harris and his associates showed that when adult chicken erythrocytes were fused with actively dividing cells, the chicken crythrocyte nuclei in the resulting heterokaryons were activated and began to synthesize both DNA and R N A (1). In these experiments only the nucleus of the chicken erythrocyte was incorporated into the heterokaryon while the cytoplasm was lost by lysis (2, 3). Erythrocyte...

Toll-like receptors mediate immune responses via recognition of pathogen-associated molecular patterns. Polymorphisms of toll-like receptors may affect their recognition of pathogen-associated molecular patterns, leading to varied host resistance to pathogenic infections. However, little is known about the polymorphisms of chicken toll-like receptors (ChTLR) among breeds. In this study, we clon...

Type VII collagen, as a major component of anchoring fibrils found at basement membrane zones, is crucial in anchoring epithelial tissue layers to their underlying stroma. Recently, type VII collagen was discovered in the inner human retina by means of immunohistochemistry, while proteomic investigations demonstrated type VII collagen at the vitreoretinal interface of chicken. Because of its po...

In a two-layer diffusing medium, polarized light directly backscattering off the superficial layer will partially retain its sense of polarization, whereas deeper-probing light will be increasingly depolarized by diffusion. This effect has been studied in both elastic scattering and fluorescence contexts. We apply this method to Raman scattering in two two-layer models with a highly diffusing l...

Non-iridescent structural plumage color is typically produced by coherent scattering of light within a matrix of keratin and air (a ;spongy layer') in feather barbs. It remains unclear what role, if any, the basal melanin layer underlying this spongy layer plays in the production of coloration. Amelanism in birds with structural color is a ;natural experiment' in which melanin pigmentation is l...

Supplementation of vitamin E and selenium improved the productivity livestock. This study aims to evaluate effect supplementation on different diets IPB D-2 chickens during 15-21 weeks. Completely randomized factorial 2 x (factor A was diet type factor B supplementation) five replications used in this experiment. The variables observed were performance, blood profile, organ immunity percentage ...

viral infections are the cause of great economic losses in the poultry industry. development of appropriate reagents and serological diagnostic kits will help to control these infections. the aim of this study is to prepare a peroxidase labeled anti-chicken igg using a mab (5b8) against chicken igg, for detection of chicken antibodies in elisa. hybridoma cells producing the mab 5b8 were culture...