newcastle disease virus (ndv) is known as one of the most important endemic viral pathogen for various avian species such as ostrich, in iran. therefore, establishing a routine vaccination program against nd in ostrich flocks would be useful in order to reduce the danger of this infection. newcastle disease occurs among the ostriches and leads to high rate of mortality while most of the losses ...

Thermostable Newcastle disease virus (NDV) vaccines have been used widely to control Newcastle disease for village poultry flocks, due to their independence of cold chains for delivery and storage. To explore the potential use of thermostable NDV as a vaccine vector, an infectious clone of thermostable avirulent NDV strain TS09-C was developed using reverse genetics technology. The GFP gene, al...

Strains of Newcastle disease virus (NDV) can be separated into genotypes based on genome differences even though they are antigenically considered to be of a single serotype. It is widely recognized that an efficacious Newcastle disease (ND) vaccine made with any NDV does induce protection against morbidity and mortality from a virulent NDV challenge. However, those ND vaccines do not protect v...

For detection of infectious laryngotracheitis virus (ILTV) antibody, glycoprotein B-, C-, and D-based enzyme-linked immunosorbent assays (B-, C-, and D-ELISAs, respectively) were developed. The B- and D-ELISAs showed enhanced detection of anti-ILTV antibodies in infected chickens compared to that of the commercial ELISA. Furthermore, the D-ELISA was efficient in detecting seroconversion with ve...

The experiments aimed to develop the inactivated Newcastle disease (ND) vaccine in the form of water in oil (WO) and water in oil in water (WOW) using palm oil, which is easily found in tropical countries, and to study the effect of vitamin E in the form of dl-α-tocopheryl acetate for improving the efficacy of inactivated ND vaccine prepared from palm oil. Two hundred and twenty, thirty five da...

in order to adapt lasota strain of newcastle disease virus (ndv) on chick embryo fibroblast (cef) cells, 0.1 ml from lasota vaccine produced in razi vaccine and serum research institute inoculated to the cef cells and was passaged five times in the cef grown in eagle’s minimum essential medium (mem). first to third passages were blind but in fourth passage cytopathic effect of virus was observe...

A Newcastle disease virus antigen quantification assay has been developed at CIDC-Lelystad as a candidate in vitro potency test for inactivated Newcastle disease vaccines. In studies performed at CIDC-Lelystad, a high correlation was demonstrated between the results of this candidate in vitro potency assay and the results of the serological potency assay (European Pharmacopoeia monograph 0870; ...

We determined the efficacy and safety of chitosan (CS)-coated poly(lactic-co-glycolic) acid (PLGA) nanoparticles (NPs) as a delivery system for a vaccine to protect chickens against Newcastle disease virus (NDV). The newly constructed vaccine contained DNA (the F gene) of NDV. The Newcastle disease virus (NDV) F gene deoxyribonucleic acid (DNA) plasmid (pFDNA)-CS/PLGA-NPs were spherical (diamet...

newcastle disease is one of the most important diseases of poultry. it usually causes a great loss inpoultry industry and domestic village chickens. since domestic chickens in villages are free ranging for food,the chance of their contact with wild birds that may act as reservoirs is high. to determine the role ofdomestic chickens in the epizootiology of newcastle disease virus in villages of i...