Purified lipopolysaccharide (LPS) extracted with phenol-water from smooth Brucella abortus was hydrolyzed with 1% acetic acid at 100 degrees C. The degraded polysaccharide (AH) released gave reactions of identity with the native polysaccharide hapten (NH) in phenol-water- or trichloroacetic acid-extracted endotoxin preparations of B. abortus and with the polysaccharide (poly B) extracted by tri...

The aim of this work was to find the most useful inorganic nitrogen source to enable Leucopaxillus giganteus to become a more nutritious mushroom, regarding organic acid and phenolic composition and total alkaloids content. For these, the influence of NH 4NO 3,NaNO 2, KNO 3,, and (NH 4) 2HPO 4 on the organic acid production was determined by HPLC-UV and total alkaloid content was assessed by a ...

A 2 × 2 factorial experiment was conducted to investigate the effect of dietary protease (0 and 200 mg/kg) and xylanase (0 and 200mg/kg) in reducing manure odor and NH(3) from finisher pigs. Sixteen pigs were assigned to 1 of 4 dietary treatments, (i) basal diet, (ii) basal diet + xylanase, (iii) basal diet + protease, or (iv) basal diet + xylanase + protease, for 24 d. The manure samples from ...

The single molecular conductance of amino acids was measured by a scanning tunneling microscope (STM) break junction. Conductance measurement of alanine gives out two conductance values at 10-1.85 G₀ (1095 nS) and 10-3.7 G₀ (15.5 nS), while similar conductance values are also observed for aspartic acid and glutamic acid, which have one more carboxylic acid group compared with alanine. This may ...

The synthesis and isolation of the sodium salt of cyclo-tri-μ-imidotetraphosphate, i.e. Na4cP4O9(NH)3·H2O, were achieved by the hydrolysis of Na4cP4O8(NH)4·2H2O under very weak acidic conditions, i.e. using 0.2 mol L(-1) propionic acid and the pH-controlled recrystallization procedure. The purity of Na4cP4O9(NH)3·H2O was improved from 2% to 95% by the pH-controlled recrystallization only two ti...

The backbone assignment of medium-sized proteins is rarely as straightforward as that of small proteins, and thus often requires creative solutions. Here, we describe the application of a combination of standard 3D heteronuclear methods with CC(CO)NH and a variety of MUltiplicity Selective In-phase Coherence transfer (MUSIC) experiments. Both CC(CO)NH and MUSIC are, in theory, very powerful met...

Diiron complexes [{(micro-SCH2)2NCH2C6H4X}{Fe(CO)2L}2] (L = CO, X = 2-Br, 1; 2-F, 2; 3-Br, 3; L = PMe(3), X = 2-Br, 4) were prepared as biomimetic models of the iron-only hydrogenase active site. The N-protonated species [(NH)]+ClO(4)(-), [(NH)](+)ClO(4)(-) and the micro-hydride diiron complex [4(FeHFe)]+PF(6)(-) were obtained in the presence of proton acids and well characterized. The protonat...

Phosphinic compounds have potential as amide-bond mimetics in the development of novel peptidomimetics, enzyme inhibitors, and metal-binding ligands. Novel pseudo-oligopeptides with two phosphinic acid groups embedded in the peptide backbone serving as amide-bond surrogates, Psi[P(O,OH)--CH(2)], were targeted. A series of linear and cyclic pseudo-oligopeptides with two phosphinic acid groups ar...

Ammonia (NH(3)) is a common exogenous gas in the atmosphere, as well as an endogenous chemical produced by amino acid catabolism and other pathways in vivo. Physiological and pathophysiological roles of NH(3) in the nervous system have been studied. Recently, endogenous NH(3) has been suggested to be a gas transmitter. However, so far the role of NH(3) in cardiovascular functions has not been r...

The cleavage of human fibrinogen in 70 % formic acid with CNBr has been studied. A fragment containing the NH%terminal portions of the three chains has been obtained from the reaction mixture. After reduction and alkylation the NH%-terminal fragments of the a(A) and a(AP) chains have been isolated in essentially pure form by gel filtration and chromatography. The amino acid sequence of these fr...