Soybean [Glycine max (L.) Merr.] is an important crop rich in vegetable protein and oil, and is a staple food for human and animals worldwide. However, soybean plants have been challenged by soybean cyst nematode (SCN, Heterodera glycines), one of the most damaging pests found in soybean fields. Applying SCN-resistant cultivars is the most efficient and environmentally friendly strategy to mana...

The common cutworm (CCW; Spodoptera litura Fabricius) is a serious herbivorous insect pest of soybean (Glycine max) in Asia and Oceania. Previously, we identified quantitative trait loci (QTLs) for CCW-antibiosis-resistance, CCW-1 and CCW-2, and antixenosis-resistance, qRslx1 and qRslx2, in the cultivar 'Himeshirazu'. The effects of these QTLs are useful in the breeding of CCW-resistant cultiva...

Three small insert (300 to approximately 600 bp) sheared genomic libraries were constructed by pipetting and DNase I treatment of soybean DNA. About 15,000 clones from each library were screened for CT- simple sequence repeats (CT-SSRs). The CT-SSRs were abundant in the soybean genome at an estimated frequency of approximately one SSR per 110 kb of genomic DNA. Following the sequencing of 129 p...

Field surveys to collect isolates of Bean pod mottle virus (BPMV), a member of the genus Comovirus, were done in 1997, 1998 and 2001 in research centers and growers’ commercial fields in Virginia. The isolates were collected by taking leaves from symptomatic plants and confirming the presence of virus using leaf blot immunoassay, followed by propagation of selected positive samples in the green...

Time to flowering in soybeans Glycine max (L.) Merr. is affected by environmental conditions, temperature and photoperiod being the leading environmental factors. Most of available experimental data in the Mississippi Valley indicate midand late spring plantings. Planting dates in soybean crops vary significantly, and late plantings are not uncommon, especially in years with extreme spring weat...

The effect o f paraq u a t on hydroxyl radical production , glutathione and ascorbate content, and glutathione reductase and superoxide dism utase activities was assayed in the to lerant “K w angkyo” and the susceptible “ H o o d ” cultivars o f soybean [Glycine max (L.) Merr.]. Seed­ lings o f both cultivars a t the fully expanded first trifoliate leaf stage were treated with paraquat at 0, 1,...

RNA sequencing (RNA-Seq) is a deep sequencing method used for transcriptome profiling. RNA-Seq assemblies have successfully been used for a broad variety of applications, such as gene characterisation, functional genomic studies, and gene expression analysis, particularly useful in the absence of a well-studied genome reference sequence. This study reports on the development of reference unigen...

We have used cDNA microarray analysis to examine changes in gene expression during embryo development in soybean (Glycine max) and to compare gene expression profiles of two soybean cultivars that differ in seed isoflavonoid content. The analysis identified 5,910 genes that were differentially expressed in both soybean cultivars grown at two different locations for two consecutive years in one ...

improvements on the efficiency of the embryogenic culture system and reduced culture time have been reA total of 15 soybean [Glycine max (L.) Merr.] cultivars representported (Samoylov et al., 1998a, 1998b; Santarém et al., ing maturity groups 00 to VIII were assessed for their embryogenic and transformation potentials. All cultivars were identified as embryo1998; Santos et al., 1997), this sys...

We used native polyacrylamide gel electrophoresis to identify polymorphism levels in α- and β-esterase loci from leaf tissues of Brazilian soybean cultivars for the analysis of population genetic diversity and structure, and to investigate relationships between conventional and genetically modified cultivars. The cultivars included lines developed by a soybean-grower cooperative (CD), by ...