نتایج جستجو برای: nine specific primer pairs for glu
تعداد نتایج: 10685704 فیلتر نتایج به سال:
Rice (Oryza sativa L.) is one of the most important crops in the world. It is essential to develop an efficient method to build the fingerprinting database of commercial hybrid rice cultivars and their parental lines in seed market for rapid and unambiguous cultivar identification and seed purity analysis as new similar cultivars increasing yearly. The objective of this investigation were to fi...
Invasive species can significantly affect local biodiversity and create important challenges for conservation. They usually present an outstanding plasticity that permits the adaptation to the new environments. Understanding their genetic background is fundamental to better comprehend invasion dynamics and elaborate proper management plans as well to infer population and evolutionary patterns. ...
The common wheat relative species are important germplasm for wheat breeding. In the present study, novel allelic variants at Glu-B3 locus were cloned to provide gene resources for wheat quality improvement. Four Glu-B3-locus specific primer sets LB1F/LB1R, LB2F/LB2R, LB3F/LB3R, and LB4F/LB4R were employed to isolate novel allelic variants of GluB3-1, GluB3-2, GluB3-3, and GluB3-4 from seven co...
almonds are self incompatible, the incompatibility being controlled by a multi allelic (s-alleles) single gene. the incompatibility alleles were studied in 96 samples of wild almonds and related prunus species from 10 taxonomic groups. polymerase chain reactions were carried out with six primer sets including: three degenerate primer pairs (paconsi-f(fam)/em-pc1consrd, paconsi-f(fam)/em-pc3cons...
The aflatoxin producer Aspergillus flavus and the koji mold Aspergillus oryzae are morphologically similar species that belong to the Aspergillus section Flavi. We used amplified fragment length polymorphism (AFLP) to differentiate these two species. In this study, we tested thirteen A. flavus, nine A. oryzae, and three A. flavus var. columnaris strains. DNA fragment profiles amplified with eac...
Wheat dough characteristics and end-use quality are strongly influenced by the amount specific composition of glutenins, major components gluten. Such proteins divided into high-molecular-weight encoded Glu-A1, Glu-B1 Glu-D1 loci; low-molecular-weight Glu-A3, Glu-B3 Glu-D3 loci. Allelic variation at each these loci has been associated with changes in wheat functionality. However, most studies c...
The chloroplastic DNA of 64 accessions of apple (Malus spp.) (54 Iranian genotypes, five commercial cultivars and five rootstocks) were analyzed to reveal their haplotypes by using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method. Approximately 4320 bp of the chloroplast genome was analyzed, by using two chloroplast universal primer pairs and two restriction ...
Botrytis cinerea is an important pathogen that causes diseases in ornamental crops. In presentresearch several greenhouses of roses located in central region of Iran were surveyed toidentify the Botrytis cinerea. A total of 80 isolates were collected from rose greenhouses incentral region of Iran. Morphological identification was based on characters such asconidiophore and conidial length. Acco...
The present case study aims to elucidate the correlation between the human 8-hydroxyguanineglycosylase (hOGG1), APE1 and X-ray repair cross-complementing gene 1 (XRCC1) gene polymorphisms to the susceptibility and clinicopathological features of primary angle closure glaucoma (PACG) in a Chinese Han population. Blood samples were obtained from 258 PACG patients (case group) and 272 healthy volu...
A three dimensional model of the basic Helix-Loop-Helix motif and its sequence specific recognition of DNA is described. The basic-helix I is modeled as a continuous ?-helix because no ?-helix breaking residue is found between the basic region and the first helix. When the basic region of the two peptide monomers are aligned in the successive major groove of the cognate DNA, the hydrophobi...
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