Giant Unilamellar Vesicles (GUVs) consisting in self-closed lipid bilayers of 0.5-100 µm diameter are considered as oversimplified models of cells because of their biological membrane and micrometric size while Large Unilamellar Vesicles (LUVs) of 100-500 nm diameter have applications in drug delivery. To improve structural and mechanical properties of these vesicles, we have developed two cate...

Healthy cells are required in large numbers to form a tissue-engineered construct and primary cells must therefore be increased in number in a process termed 'expansion'. There are significant problems with existing procedures, including cell injury and an associated loss of phenotype, but three-dimensional culture has been reported to offer a solution. Reversible gels, which allow for the reco...

Thermosensitive molecularly imprinted polymers (T-MIPs) on porous carriers were prepared via the synergy of dual functional monomers of 4-vinylpyridine (VP) and N-isopropylacrylamide (NIPAM), for selective recognition and controlled adsorption and release of bisphenol A (BPA) by the temperature regulation. The porous polymer supporter was synthesized by multistep swelling of polystyrene and the...

Among the natural macromolecules potentially used as the scaffold material in hydrogels, xylan has aroused great interest in many fields because of its biocompatibility, low toxicity, and biodegradability. In this work, new pH and thermoresponsive hydrogels were prepared by the cross-linking polymerization of maleic anhydride-modified xylan (MAHX) with N-isopropylacrylamide (NIPAm) and acrylic ...

Surface-immobilized poly(N-isopropyl acrylamide) (pNIPAM) is currently used for a wide variety of biosensor and biomaterial applications. A thorough characterization of the surface properties of pNIPAM thin films will benefit those applications. In this work, we present analysis of a plasma-polymerized NIPAM (ppNIPAM) coating by multiple surface analytical techniques, including time-of-flight s...

A new family of linear polymers with pronounced affinity for arginine- and lysine-rich proteins has been created. To this end, N-isopropylacrylamide (NIPAM) was copolymerized in water with a binding monomer and a hydrophobic comonomer using a living radical polymerization (RAFT). The resulting copolymers were water-soluble and displayed narrow polydispersities. They formed tight complexes with ...

The main objective of this study is to prepare, thermally, sensitive microgel particles bearing thiol groups via precipitation polymerization of N-isopropylacrylamide (NIPAM), methylenebisacrylamide (MBA) and vinylbenzylisothiouronium chloride (VBIC) using 2-2'-azobis(2-amidinopropane)-dihydrochloride (V50) as initiator. The influence of various parameters has been investigated as a systematic ...

Materials Tris[2-(dimethylamino)ethyl]amine (Me6TREN) was prepared as described in the literature. Oligo (ethylene glycol) methacrylate with an average Mn of 500 g/mol (OEGMA-OH) was purchased from Sigma-Aldrich and filtered through basic alumina to remove stabilizers. N-Isopropylacrylamide (NIPAM) was recrystallized from cyclohexane before use. Cuprous bromide (CuBr) and cuprous chloride (CuCl...