We investigated the use of DNA amplification by polymerase chain reaction (peR) for detection of Mycobacterium tuberculosis in 300 patients who were suspected of having pulmonary tuberculosis and compared the results with culture results which were performed in parallel with PCR. Two-thirds of each sample was processed for smear and culture by standard methods and one-third was prepared fo...

AbstractChronic sialadenitis is a disease of salivary glands associated with chronic infections,systemic diseases and silolithiasis pathogenesis of the disease. Primary tuberculoussubmandibulitis is not a reported disease. The diagnosis was confirmed whena 45 years old lady suspicious to submandibular gland tumor was operated on for excisionalbiopsy. Histological examination of the specimen by ...

The potentially pathogenic Non-Tuberculosis Mycobacteria (NTM) are emerging nowadays which result in pulmonary and non-pulmonary infections in human. This group of bacteria consists of at least 200 different species. While the pulmonary disease is the most common form of NTM infections, NTM can cause diffused infections as well as extrapulmonary infections in every organ, such as bone marrow, s...

background and objectives: tuberculosis (tb) is the oldest known bacterial disease in humans. due to the rise of morbidity in recent years, early diagnosis of the disease is necessary. materials and methods: in this study we used fluorescent amplification–based specific hybridization (flash) pcr to target is6110 for rapid detection of m. tuberculosis (mtb). to investigate the important factors ...

The current study aimed to evaluate the feasibility of using aminoglycoside 2″-O-phosphotransferase aph(2″) gene as a positive selection marker in N. asteroides, M. smegmatis, abscessus and tuberculosis. gene, known confer resistance tobramycin, was PCR amplified from farcinica cloned into two plasmid vectors, pMSG383 pDB151, harboring hygromycin zeocin markers, respectively. recombinant plasmi...

background pe5 is a member of the pe protein family whose precise function is yet to be understood. there are about 100 members of the pe family proteins in mycobacterium tuberculosis. these glycine- and alanine-rich proteins consist of proline-glutamate motifs at their n-termini and may be implicated in pathogenesis of the bacilli. objectives we aimed to clone and over-express the rv0285 codin...

1 Boiron P, Provost F, Chevier G, Dupont B. Review of nocardial infections in France 19871990. Eur J Clin Microbiol Infect Dis 1992; 11:709-14. 2 Chen CJ. Nocardia asteroides endophthalmitis. Ophdialmic Surg 1983;14:502-5. 3 Dewsnup DH, Wright DN. In vitro susceptibility of Nocardia asteroides to 25 antimicrobial agents. Anmimrob Agent Chemother 1984;25: 165-7 4 Douglas RM, Grove DI, Elliott J,...