The magnitude of demand for planting materials in grape, mainly for rootstock genotypes indicates that micropropagation is inevitably necessary for their mass scale propagation. Therefore, the studies on micropropagation of four genetically different grape rootstocks namely Dogridge (Vitis champini), SO4 (V. riparia× V. berlandieri), H-144 (V. vinifera × V. labrusca) and 3309 C (V. riparia × V....

Citrullus colocynthis, belonging to the family of cucurbitaceae. It is a xerophytic desert plant, widely used for its medicinal purpose. In tribal areas it is used for the treatment of constipation, oedema, bacterial infection, cancer, diabetes and abortifacient. Present study is based on developing a protocol for plantlet regeneration from the nodal segment. The sterilized explants were inocul...

Colchicine was used as chemical mutagen for In vitro induction of polyploids. Nodal segments of In vitro grown marigold plantlets were cultured on MS medium supplemented with four different concentrations of colchcine (0.0%, 0.001%, 0.01% and 0.05%) for 12h, separately. After treatment, nodal segment were shifted to colchicine free MS medium for normal growth. Highest mortality rate (72.74%) an...

An efficient protocol was established for in vitro plant regeneration of Paederia foetada L. (Family. Rubiaceae), a widely used medicinal vine of Bangladesh through callus culture in using nodal segment. Yellowish green nodular callus was observed from nodal segments on MS basal medium supplemented with 1.5 mg/L BAP + 0.5 mg/L NAA within three weeks. Large number of shoots (14.4±1.29) were obta...

In this study, several methods have been used to facilitate shoot formation from nodal explants of local almond ecotypes known as “Beldi” grown in Eastern Morocco. Nodal segments divers old genotypes were cultured on various concentrations auxin (indole-3-butyric acid (IBA)) and cytokinins (6-benzyl-aminopurine (BAP), thidiazuron (TDZ), kinetin (KIN)) added two different media (Murashige Skoog ...

1. Asparagus racemosus is an herb used as a rasayana in Ayurveda. The root of A racemosus is popular for its various medicinal uses such as appetizer, stomachic, tonic aphrodisiac galactogogus etc. In this experiment we have found the shoot induction after 3 weeks when Nodal explants segments were cultured in basal medium supplemented with BAP, Kin and Ads and Shoots induction on MS basal mediu...

Indian citrus ringspot virus (ICRSV) is known to cause serious problem in Kinnow (Citrus nobilis Lour × C. deliciosa Tenora). This paper reports the elimination of ICRSV from Kinnow by chemotherapy coupled with shoot tip grafting under in vitro conditions. Nodal segments from infected mother plant (indexed by indirect ELISA and RT-PCR) were cultured on MS medium containing 2-iP (1mg/l) and malt...

In vitro propagation through nodal explants by indirect organogenesis of Clinacanthus siamensis Bremek. Nodal segments were surface sterilised in NaOCl for 5 min. This is followed by treating it with 0.1% Hgcl2 for 8 min. Different concentrations of BAP and 2P were added to full strength MS medium for culture initiation. Multiple shoots were formed in MS medium augmented with various concentrat...

Pterocarpus santalinus belongs to fabaceae family and is one of the endemic and endangered medicinal plant species of seshachalam hills, Tirumala, Andhra Pradesh state, India. In the present study we have successfully demonstrated the in vitro regenartion of this plant through shoot bud culture. The seeds germinated sequentially on plastic trays and transferred polythene bags for 21 days served...