نتایج جستجو برای: nuclear reprogramming
تعداد نتایج: 249719 فیلتر نتایج به سال:
Transplantation of Xenopus laevis cell nucleus to enucleated Xenopus egg leads to the generation of cloned animal. This exemplifies the process of nuclear reprogramming by which the nucleus of a specialized cell is reset to an embryonic state from which it can generate all the cells of an organism. Using the precursor of the egg, the oocyte, it is also possible to reprogram somatic cell. The ad...
Impaired development of embryos produced by somatic cell nuclear transfer (SCNT) is mostly associated with faulty reprogramming of the somatic nucleus to a totipotent state and can be improved by treatment with epigenetic modifiers. Here we report that addition of 100 μM vitamin C (VitC) to embryo culture medium for at least 16 h post-activation significantly increases mouse blastocyst formatio...
Reprogramming of somatic cells achieved by combination of the four transcription factors Oct4, Sox2, Klf4, and c-Myc has very low efficiency. To increase the reprogramming efficiency and better understand the process, we sought to identify factors that mediate reprogramming with higher efficiency. We established an assay to screen nuclear fractions from extracts of pluripotent mouse cells based...
The ability to reprogram somatic cell nuclei back into a pluripotent epigenetic state provides exciting new possibilities for in vitro research and cell transplantation therapy. There has been a significant quantity of recent research studies demonstrating that this epigenetic reprogramming process is possible with human and non-human primate somatic cells. In this review, various methodologies...
Somatic cell nuclear transfer and transcription factor mediated reprogramming are two widely used techniques for somatic cell reprogramming. Both fully reprogrammed nuclear transfer embryonic stem cells and induced pluripotent stem cells hold potential for regenerative medicine, and evaluation of the stem cell pluripotency state is crucial for these applications. Previous reports have shown tha...
Background: Since the sheep ‘Dolly’ was successfully cloned by somatic cell nuclear transfer (SCNT), the inefficiency of SCNT cloning and abnormality of cloned animals have been a focus of considerable controversy. Oocyte quality has been shown to contribute to poor somatic cloning and cell reprogramming efficiency with many factors affecting oocyte quality and developmental potential. It has b...
Nuclear transfer to oocytes is an efficient way to transcriptionally reprogram somatic nuclei, but its mechanisms remain unclear. Here, we identify a sequence of molecular events that leads to rapid transcriptional reprogramming of somatic nuclei after transplantation to Xenopus oocytes. RNA-seq analyses reveal that reprogramming by oocytes results in a selective switch in transcription toward ...
Research on somatic cell reprogramming has progressed significantly over the past few decades, from nuclear transfer into frogs' eggs in 1952 to the derivation of human-induced pluripotent stem (iPS) cells in the present day. In this article, I review five landmark papers that have laid the foundation for current efforts to apply somatic cell reprogramming in the clinic.
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