Thermodynamic features related to preparation and use of self-assemblies formed between multilamellar and unilamellar zwitterionic liposomes and polynucleotides with various conformation and sizes are presented. The divalent metal cation or surfactant-induced adsorption, aggregation and adhesion between single- and double-stranded polyribonucleotides and phosphatidylcholine vesicles was followe...

Thermodynamic features related to preparation and use of self-assemblies formed between multilamellar and unilamellar zwitterionic liposomes and polynucleotides with various conformation and sizes are presented. The divalent metal cation or surfactant-induced adsorption, aggregation and adhesion between single- and double-stranded polyribonucleotides and phosphatidylcholine vesicles was followe...

Protein-nucleic acid interaction plays an essential role in the regulation of gene expression. Structural and binding information provides insight into the mechanism of protein-nucleic acid recognition. Although there are structural databases for protein-nucleic acid complexes, there exists no database for protein-nucleic acid binding. Thus, we have decided to develop the Thermodynamic Database...

Cationic liposome-nucleic acid complexes, which were originally developed for use as non-viral gene delivery vectors, may now have an equally important application as immunotherapeutic drugs. Recent studies have highlighted the ability of cationic liposomes to potently activate the innate immune system when used to deliver certain Toll-like receptor (TLR) agonists. The immune-enhancing properti...

MOTIVATION There are many important proteins which bind single-stranded nucleic acids, such as the nucleocapsid protein in HIV and the RecA DNA repair protein in bacteria. The presence of such proteins can strongly alter the secondary structure of the nucleic acid molecules. Therefore, accurate modeling of the interaction between single-stranded nucleic acids and such proteins is essential to f...

Nucleic acid detection in hematological disorders has been carried out in a wide range of medical fields or departments. Hence, it is recommended that the hematology laboratory should draw up guidelines or a manual for each disease (group), field or specialty. Nucleic acid detection is defined as processes which are disclosed using depolymerization of a nucleic acid hybrid (deoxyribonucleic aci...

The yeast nucleic acid used in these experiments was a commercial preparation purified by treatment with picric acid (3). A sample of yeast nucleic acid and one of thymus nucleic acid were kindly supplied by Dr. P. A. Levene to whom the author wishes to extend his heartiest thanks. The effect of the enzyme preparations on nucleic acid was followed by determining the amount of total phosphorus r...

A trace lead ion (Pb 2+ ) detection platform combining surface-enhanced Raman spectroscopy (SERS) with nucleic acid cycle multiple signal amplification.

Nucleic acid nanostructures are versatile and nearly infinitely customizable, which can make it challenging to find the right one.

BACKGROUND Multiplexing technologies, which allow for simultaneous detection of multiple nucleic acid sequences in a single reaction, can save a lot of time, cost and labor compared to traditional single reaction detection methods. However, the multiplexing method currently used requires precise handiwork and many complicated steps, making a new, simpler technique desirable. Oligonucleotides co...