MOTIVATION The increasing use of customized oligo-DNA-microarrays has created a demand for convenient design of oligo nucleotides. The oligo-nucleotides have to serve as specific templates in the hybridization reaction. RESULTS We have devised a web-based system for interactive design of suitable oligos for transcription profiling experiments with user-defined parameters. Oligodb is based on ...

When plasmid DNA duplexes carrying the regular homopurine-homopyrimidine inserts (dGA)n, (dTC)n and (dG)n, (dC)n are preincubated with homologous labeled oligo(dPy) ((dTC)n and (dC)n respectively) at acid pH, the label co-electrophoreses with the duplex DNA. Thus, a very strong complex is formed. Complementary oligo(dPu) does not form a complex under these conditions. No binding is observed for...

Differences in the frequencies of chemical words of a given length in two nucleic sequences are used to define an “oligo-distance” between the sequences. Oligo-distances are much easier and faster to compute than the distances conventionally determined by sequence alignment. A correlation between oligo-distance and alignment-distance is observed. The two kinds of distances are used to construct...

Evaluation of the efficacy of blue-green algal compounds against the growth of either pathogenic or antagonistic microorganisms as well as their effect on the antagonistic ability of bioagents was studied under in vitro conditions. The present study was undertaken to explore the inhibitory effect of commercial algal compounds, Weed-Max and Oligo-Mix, against some soil-borne pathogens. In growth...

Biostratigraphy of Oligo-Miocene marine deposits Central Iran basin based on calcareous nannoplanktons

A novel beta-cyclodextrin (beta-CD) derivative modified with a degradable and biocompatible oligo(lactic acid) (OLA) group, 6-oligo(lactic acid)cyclomaltoheptaose (6-OLA-beta-CD), was successfully synthesized and used as a chiral selector for the capillary electrophoretic (CE) resolution (R(s)) of several basic analytes. The primary purpose of the research was to explore the capability of the 6...

A template-dependent RNA polymerase has been isolated from poliovirus-infected cells by assaying for the ability of the enzyme to copy poly(A) complexed to an oligo(U) primer. The polymerase was solubilized with detergent, and RNA was removed by precipitation with 2 M LiCl. The solubilized polymerase required both poly(A) and oligo(U) for activity and was stimulated by Mg2+ but was inhibited by...

Tissue reconstruction has an unmet need for soft active scaffolds that enable gentle loading with regeneration-directing bioactive components by soaking up but also provide macroscopic dimensional stability. Here microporous hydrogels capable of inverse shape-memory effect (iSME) are described, which in contrast to classical polymers (SMPs) recover their permanent shape upon cooling. These desi...

This paper describes the technology and performance characteristics of the Oligo GEArray® DNA microarray system. Oligo GEArrays® are an effective alternative to genome-wide microarrays, in that each array is designed to profile expression of a focused gene panel from a particular biological pathway or disease state. The data presented in this paper substantiates the sensitivity, reproducibility...