Oligonucleotide-templated reactions are useful for applying nucleic acid sensing. Various chemistries for oligonucleotide-templated reaction have been reported so far. Major scientific interests are focused on the development of signal amplification systems and signal generation systems. We introduce the recent advances of oligonucleotide-templated reaction in consideration of the above two poi...

Methodology is described for the synthesis of DNA oligomers containing a free 3'-thiol group which can be selectively crosslinked with a wide variety of probes. This chemistry is compatible with both phosphotriester and phosphoramidite solid phase chemistry. Moreover, the sulphydryl group is introduced into the 3'-nucleoside solid support linkage prior to oligonucleotide synthesis. Consequently...

An improved physico-chemical model of hybridization on high-density oligonucleotide microarrays .. 5 Comparison of Affymetrix GeneChip expression measures .................................................................... 5 Evaluation of methods for oligonucleotide array data via quantitative real-time PCR............................ 5 Gene expression levels assessed by oligonucleotide microa...