نتایج جستجو برای: oocyte fertilization
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The oocyte maturation includes nuclear maturation and cytoplasmic maturation before fertilization. Moreover, the oocyte nucleus, the cytoplasm and other cell organelles occur changes, zona pellucida (ZP) synchronously undergoes biochemical and structural modifications in the final maturation phase of oocyte prior to fertilization (ZP maturation). It is reported that ZP modifications, such as gl...
The oocyte is a highly differentiated cell. It makes organelles specialized to its unique functions and progresses through a series of developmental stages to acquire a fertilization competent phenotype. This review will integrate the biology of the oocyte with what is known about oocyte-specific gene regulation and transcription factors involved in oocyte development. We propose that oogenesis...
Fertilization is the fusion of the male and female gamete. The process involves the fusion of an oocyte with a sperm, creating a single diploid cell, the zygote, from which a new individual organism will develop. The elucidation of the molecular mechanisms of fertilization has fascinated researchers for many years. In this review, we focus on this intriguing process at the molecular level. Seve...
One of the biggest prerequisites for pregnancy is the fertilization step, where a human haploid spermatozoon interacts and penetrates one haploid oocyte in order to produce the diploid zygote. Although fertilization is defined by the presence of two pronuclei and the extraction of the second polar body the process itself requires preparation of both gametes for fertilization to take place at a ...
Single oocyte manipulation in microfluidic channels via precisely controlled flow is critical in microfluidic-based in vitro fertilization. Such systems can potentially minimize the number of transfer steps among containers for rinsing as often performed during conventional in vitro fertilization and can standardize protocols by minimizing manual handling steps. To study shape deformation of oo...
With the introduction of intracytoplasmic sperm injection (ICSI), couples with severe male factor infertility have achieved fertilization and clinical pregnancy rates comparable to other in-vitro fertilization (IVF) patients. However, failure of fertilization still occurs in some patients despite the utilization of microsurgical sperm injection techniques. How such fertilization failure after I...
Background Failed fertilization occurs 1-3% in the infertile couples. Failure of oocyte activation (OA) is mainly attributed to failed fertilization. ICSI alone artificial oocyte activation (AOA) is only treatment approach for these individuals. Three procedures are introduced for AOA: chemical, mechanical and electrical. Most of the clinical Centers have commonly used ionomycin for activation ...
This is a questionnaire based study of 501 women enquiring about anonymous oocyte donation at a private in-vitro fertilization (IVF) unit, investigating the demographic characteristics and logistic issues involved in ovum donation. The 501 women were made up of 356 women who did not donate ('non-donors') and 145 women who eventually donated their oocytes ('donors'). Although there was a majorit...
BACKGROUND A successful oocyte cryopreservation programme is of utmost importance where a limited number of oocytes can be inseminated per cycle, to overcome legal and ethical issues related to embryo storage, for oocyte donation programmes and for fertility preservation (especially for cancer patients). Vitrification has been recently proposed as an effective procedure for this purpose. METH...
We investigated the relationship between oxidative stress and poor oocyte quality and whether the antioxidant melatonin improves oocyte quality. Follicular fluid was sampled at oocyte retrieval during in vitro fertilization and embryo transfer (IVF-ET). Intrafollicular concentrations of 8-hydroxy-2'-deoxyguanosine (8-OHdG) in women with high rates of degenerate oocytes were significantly higher...
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