Background: The aim of this study was to compare two vitrification systems (Cryotop and OPS) on mouse MII oocytes and evaluate its effects on expression of heat shock protein A1A and MnSOD genes. Materials and Methods: Groups of about 15 oocytes without cumulus complexes (granulose, et. al.) were collected and exposed to equilibration and vitrification solutions before loading the oocytes into ...

Objective(s) Cigarette and nicotine enhances embryogenesis, fertility, pregnancy loss and ultrastructure alterations of oocyte. This study was performed to determine the effect of daily supplementation of vitamin E on oocytes apoptosis in nicotine-treated mice. Materials and Methods In this experimental study, 24 NMARI adult female mice were randomly allocated into four experimental groups. ...

Introduction: In vitro maturation and development of immature oocytes, as an artificial reproduction technique, is useful especially in women who are affected by cancer and polycystic ovary syndrome. Despite using many types of in vitro media, an appropriate environment has not been reported yet. Present study was designed to assess the effect of heated human follicular fluid (hHFF), which is s...

Background Polycystic ovarian syndrome (PCOs) is one of the most common causes of infertility in women of reproductive age. Currently, one of the best therapeutic options for PCOs patients is ICSI. Moreover, IVM can be a useful technique for women with PCOs who are at the risk of ovarian hyperstimulation syndrome (OHSS). On the other hand, the oocyte quality can be a determining factor for outc...

OBJECTIVE We evaluated the fertilization potential of immature oocytes obtained from controlled ovarian hyperstimulation cycles of patients undergoing ICSI. METHODS We retrospectively analyzed 463 ICSI cycles containing at least one immature oocyte at oocyte denudation. ICSI was performed on mature oocytes at oocyte denudation (metaphase-II [MII] oocytes) and the oocytes that extruded the fir...

background: the utilization of immature oocytes derived from stimulated cycles could be of great importance, specially for urgent fertility preservation cases. the aim of the current study was to determine whether in-vitro maturation is more successful before or after the vitrification of these oocytes. materials and methods: the study was performed in a private ivf center. 318 germinal vesicle...

BACKGROUND Fertilization failure is one of the most problems in assisted reproduction technology (ART). OBJECTIVE The aim of this study was the evaluation of oocytes activation by addition of calcium ionophore in unfertilized oocytes in ICSI cycles. MATERIALS AND METHODS This study was done on 15 ICSI cycles (stimulated with standard long protocol). Mature retrieved oocytes with normal morp...

ObjectiveTo appraise the fertilization rate as a predictive factor for cumulative live birth (CLBR).DesignMulticenter retrospective cohort study.SettingTen in vitro clinics, whose data were collected and processed by assisted reproductive technology (ART) Italian National Registry.Patient(s)7,968 couples undergoing 9,394 complete intracytoplasmic sperm injection cycles.Intervention(s)None.Main ...

OBJECTIVE To investigate the presence of programmed cell death in unfertilized oocytes after intracytoplasmic sperm injection (ICSI), assuming that previous apoptotic events could be correlated with the fertilization failure. DESIGN Comparison of the rate of DNA fragmentation in human oocytes at different stages of maturation soon after pick-up (control) and in unfertilized oocytes after ICSI...

In vitro growth culture systems for oocytes are being developed in several mammalian species. In these growth culture systems, in vitro grown oocytes usually have lower blastocyst formation than in vivo grown oocytes after in vitro fertilization. Furthermore, there have been a few reports that investigated the fertilization ability of in vitro grown oocytes in large animals. The purpose of this...