Organ cultures of human embryonic trachea in test tubes were used as an adjunct to tissue cultures in the isolation of respiratory viruses from children in hospital. Fifty-one viruses were obtained from 127 specimens, giving an isolation rate of 40%. Fifteen viruses were isolated from the original tissue cultures and also after passage through organ culture. Thirty viruses were isolated from th...

Plants are valuable sources of a vast array of chemical compounds like alkaloids, flavonoids, pigments, antimicrobials, pharmaceutical, etc. The majority of the compounds were produced by plants during different stages of cell, tissues and organ differentiation. Plant tissue culture is the process by which parts of plant can be grown in vitro condition in a sterile culture medium. The nutrient ...

Despite recent advances in tissue culture methodology, only 20 to 35 per cent of adults with acute upper respiratory tract illness yield viruses which can be cultivated by the standard virus recovery techniques.3' 4, 12 Recently, an increase in virus isolation from patients with common colds was achieved by Tyrrell and Bynoe through the use of human embryonic tracheal and nasal organ cultures.9...

Dental scientists are eagerly embracing regenerative medicine using tissue engineering technology. Enamel, one component of teeth, is the hardest tissue in the body. It cannot regenerate by itself, because it is formed by a layer of ameloblasts that is lost by the time the tooth crown has completely formed. Although the enamel is spent during lifetime, it is vulnerable to wear, damage, and deca...

Primary culture of human hepatocytes is an in vitro model widely used to investigate numerous aspects of liver physiology and pathology. The technique used to isolate human hepatocytes is based on two-step collagenase perfusion. Originally performed in situ for obtaining hepatocytes from the adult rat, this technique has been adapted to the ex vivo treatment of human liver from organ donors or ...

Testing cardiac gene and cell therapies in vitro requires a tissue substrate that survives for several days in culture while maintaining its physiological properties. The purpose of this study was to test whether culture of intact cardiac tissue of neonatal rat ventricles (organ explant culture) may be used as a model to study gene and cell therapy. We compared (immuno) histology and electrophy...

Introduction: It is estimated that 100 - 300 million people in the world are infected with Strongyloides stercoralis. Although there are several laboratory diagnostic methods for detection of this parasite, there is still no gold standard diagnostic method. The need for an application test with high sensitivity and specificity is highly felt, especially in people with immune deficiency or organ...