SUMMARY forqs is a forward-in-time simulation of recombination, quantitative traits and selection. It was designed to investigate haplotype patterns resulting from scenarios where substantial evolutionary change has taken place in a small number of generations due to recombination and/or selection on polygenic quantitative traits. AVAILABILITY AND IMPLEMENTATION forqs is implemented as a comm...

Methods Post-hoc analysis of a prospective observational cohort from a previous study on AKI conducted in our Unit. We defined AKI based in KDIGO II/III stages criteria. We calculated a PS for patients with or without diuretics by regression analysis and pairing 1:1 by quintiles grouping. Then performed an analysis for diuretic-AKI and diuretic-mortality by logistic regression, including the PS...

Odontoblast differentiation during physiological and reparative dentinogenesis is dependent upon multiple signaling molecules, including fibroblast growth factors (FGFs), bone morphogenetic proteins (BMPs) and Wingless/Integrated (Wnt) ligands. Recent studies in our laboratory showed that continuous exposure of primary dental pulp cultures to FGF2 exerted biphasic effects on the expression of m...

With regard to the bone-regenerative capacity, bone marrow stromal cells (BMSC) can still be termed the "gold standard." Nevertheless, neonatal stromal cells from cord blood (CB) feature advantages concerning availability, immaturity, and proliferation potential. The detailed gene expression analysis and overexpression of genes expressed differentially provide insight into the inherent capacity...

Caldicellulosiruptor kronotskyensis grows on lignocellulosic biomass by the catalysis of intrinsic glycoside hydrolase, and has potential application for consolidated bioprocessing. In current study, two predicted extra- (Xyn10A) and intracellular (Xyn10B) xylanase from C. kronotskyensis were comparatively characterized. Xyn10A and Xyn10B share GH10 catalytic domain with similarity of 41%, whil...

OBJECTIVES Oestrogen has been proven to significantly enhance osteogenic potency, while oestrogen deficiency usually leads to impaired osteogenic differentiation of mesenchymal stem cells. However, little is known concerning direct effects of oestrogen on differentiation of human dental pulp stem cells (DPSCs). MATERIALS AND METHODS In this study, human DPSCs were isolated and treated with 10...

The aim of this study was to investigate the biological function of SHANK2 on the osteo/dentinogenic differentiation potentials of human stem cells from apical papilla (SCAPs). Real-time RT-PCR was used to detect the expression of SHANK2 in human mesenchymal stem cells (MSCs). Small hairpin RNA (shRNA) was used to knockdown the SHANK2 in SCAPs. The knockdown efficiency was determined by real-ti...

OBJECTIVE To study the effect and mechanism of geraniin on the osteogenesis of tumour necrosis factor-alpha (TNF-α)-induced bone marrow-derived mesenchymal stem cells (BMSCs). METHODS BALB/c mice aged 4-6 weeks were used to collect BMSC. Methyl thiazol tetrazolium assay and lactate dehydrogenase assay were used to analyse the effect of geraniin on the TNF-α-induced impairments of osteogenesis...

We have measured the total system latencies of MacOS 10.2.8, Red Hat Linux (2.4.25 kernel with low-latency patches), and Windows XP from stimulus in to audio out, with stimuli including analog and digital audio, and the QWERTY keyboard. We tested with a variety of audio hardware interfaces, audio drivers, buffering and related configuration settings, and scheduling modes. All measured audio lat...

The aim of the present study was to elucidate the role of osteoblasts in bisphosphonates-related osteonecrosis of the jaw (BRONJ). The specific objective was to evaluate the effect on osteoblasts of two nitrogen-containing BPs (zoledronate and alendronate) and one non-nitrogen-containing BP (clodronate) by analyzing modulations in their expression of genes essential for osteoblast physiology. R...